Zeng Ye, Liu Jie, Cheng Zhichao, Zheng Sirong, Zhang Hanrong, Zhou Jueyu
Department of Stomatology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
Department of Gynecology and Obstetrics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2018 Jul 30;38(7):794-799. doi: 10.3969/j.issn.1673-4254.2018.07.04.
To investigate the alterations in gene expression profiles of cervical cancer cell line SiHa over-expressing miR-519d.
SiHa cells were transfected with a miR-519d mimic or a negative control (NC) and the changes in gene expression profiles were examined using NimbleGen human gene expression microarray. Bioinformatics approaches based on the microarray data were used to identify the targeted genes of miR-519d. Real-time quantitative PCR was employed to confirm the expression of the potential target genes.
A total of 5172 genes were found to be differentially expressed in SiHa cells over-expressing miR-519d, including 2476 up-regulated and 2796 down-regulated genes. We identified 164 potential target genes of miR-519d, and their functions were predicted using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) biological pathway analysis, and STRING database and pSTIING were used to search the key nodes in the protein-protein interactions and transcriptional regulatory networks in cancer. Real-time quantitative PCR confirmed the differential expressions of several candidate target genes.
Over-expression of miR-519d alters gene expression profiles in SiHa cells. The 164 target genes of miR-519d we identified may provide insights into the role of miR-519d in cervical tumorigenesis.
研究过表达miR-519d的宫颈癌SiHa细胞系基因表达谱的变化。
用miR-519d模拟物或阴性对照（NC）转染SiHa细胞，采用NimbleGen人类基因表达微阵列检测基因表达谱的变化。基于微阵列数据的生物信息学方法用于鉴定miR-519d的靶基因。采用实时定量PCR验证潜在靶基因的表达。
共发现5172个基因在过表达miR-519d的SiHa细胞中差异表达，其中上调基因2476个，下调基因2796个。我们鉴定了164个miR-519d的潜在靶基因，并利用基因本体论（GO）和京都基因与基因组百科全书（KEGG）生物途径分析预测其功能，使用STRING数据库和pSTIING搜索癌症中蛋白质-蛋白质相互作用和转录调控网络中的关键节点。实时定量PCR证实了几个候选靶基因的差异表达。
miR-519d的过表达改变了SiHa细胞的基因表达谱。我们鉴定的164个miR-519d靶基因可能为深入了解miR-519d在宫颈肿瘤发生中的作用提供线索。
