与pKM101相比，新型R质粒pEB017在大肠杆菌野生型、recA和uvrA菌株中的表达。

Expression of a novel R-plasmid pEB017 compared to pKM101 in Escherichia coli wild-type, recA and uvrA strains.

作者信息

Obaseiki-Ebor E E, Akerele J O

机构信息

Department of Pharmaceutical Microbiology, University of Benin, Nigeria.

出版信息

Mutat Res. 1987 Nov;192(3):175-80. doi: 10.1016/0165-7992(87)90052-2.

DOI:10.1016/0165-7992(87)90052-2

PMID:3317032

Abstract

The expression of bacterial resistance to UV irradiation and nitrofurantoin by a novel R-plasmid pEB017 in DNA-repair-proficient (wild-type) and -deficient (recA; uvrA) host strains was compared to the effects of plasmid pKM101 in the isogenic strains. pEB017 partially protected the uvrA strain, and completely protected the wild-type and recA strains from the killing effect of UV irradiation; pKM101 had no effect on the recA strain and only enhanced the survival of the wild-type and the uvrA strains after UV irradiation. pEB017 conferred nitrofurantoin resistance 10-fold on the wild-type and the recA strains and 4-fold on the uvrA strain; pKM101 did not confer nitrofurantoin resistance on the wild-type and recA strains but gave 4-fold resistance in the uvrA strain.

摘要

将新型R质粒pEB017在DNA修复功能正常（野生型）和缺陷（recA；uvrA）宿主菌株中对紫外线照射和呋喃妥因的抗性表达，与质粒pKM101在同基因菌株中的作用效果进行了比较。pEB017部分保护uvrA菌株，并完全保护野生型和recA菌株免受紫外线照射的杀伤作用；pKM101对recA菌株无影响，仅能提高野生型和uvrA菌株在紫外线照射后的存活率。pEB017使野生型和recA菌株对呋喃妥因的抗性提高10倍，使uvrA菌株提高4倍；pKM101未使野生型和recA菌株产生呋喃妥因抗性，但使uvrA菌株产生4倍抗性。