Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, Sichuan, PR China.
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Int J Syst Evol Microbiol. 2020 Dec;70(12):6450-6457. doi: 10.1099/ijsem.0.004557.
A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204, was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach. The cell-wall peptidoglycan contained ornithine as the diagnostic diamino acid. 16S rRNA gene sequence analysis indicated that strain W204 was closely related to CPCC 203535 (97.4 %, similarity), CGMCC 4.5582 (96.9 %), GP-T3-3 (96.8 %), JLT9 (96.7 %), CPCC 203383 (96.6 %) and K22-20 (96.6 %). However, the digital DNA-DNA genome hybridization value between strain W204 and the closest related strain CPCC 203535 was 21.90 %. Complete genome analyses revealed that the size of the genome was 3.54 Mb and the genomic DNA G+C content was 70.79 mol%. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid. The major menaquinone was MK-8(H). The predominant cellular fatty acids were iso-C, anteiso-C and C. The phenotypic, chemotaxonomic and phylogenetic data suggested that strain W204 should be classified as representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is W204 (=GDMCC 1.1391=KCTC 49237).
一株新的革兰氏阳性、黄色、短杆状或球形细菌菌株 W204 是从中国贾家登峪国家森林公园的土壤样本中分离出来的,并用多相方法进行了表征。细胞壁肽聚糖含有鸟氨酸作为诊断二氨基酸。16S rRNA 基因序列分析表明,菌株 W204 与 CPCC 203535(97.4%,相似度)、CGMCC 4.5582(96.9%)、GP-T3-3(96.8%)、JLT9(96.7%)、CPCC 203383(96.6%)和 K22-20(96.6%)密切相关。然而,菌株 W204 与最接近的相关菌株 CPCC 203535 之间的数字 DNA-DNA 基因组杂交值为 21.90%。全基因组分析表明,基因组大小为 3.54Mb,基因组 DNA G+C 含量为 70.79mol%。极性脂谱由二磷酸甘油酯、磷脂酰甘油、磷脂酰胆碱、一种未鉴定的糖脂、一种未鉴定的磷脂和一种未鉴定的脂组成。主要的menaquinone 是 MK-8(H)。主要的细胞脂肪酸是 iso-C、anteiso-C 和 C。表型、化学分类和系统发育数据表明,菌株 W204 应被归类为属的一个新种的代表,建议将其命名为 sp. nov.。模式菌株为 W204(=GDMCC 1.1391=KCTC 49237)。