Department of Thoracic Surgery, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
Department of Neurology, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.
Mol Med Rep. 2021 Jan;23(1). doi: 10.3892/mmr.2020.11650. Epub 2020 Nov 12.
SH3 domain‑containing kinase‑binding protein 1 (CIN85), an 85 kDa protein known to be a member of the signal adaptor family, is abnormally expressed in several human malignancies and has been found to be involved in the growth, migration and invasion of these tumors. The objective of the present study was to clarify the clinical significance of CIN85 in human esophageal squamous cell carcinoma (ESCC), as well as its in vitro functions. CIN85 expression was evaluated in 129 cases of ESCC and its adjacent normal tissues using immunohistochemistry to explore its clinical relevance and prognostic value. The functions of CIN85 in the ESCC TE1 cell line were analyzed in vitro using the interfering short hairpin RNA silencing technique. MTS, wound healing, clone formation and Transwell assays were used to detect the proliferation, migration and invasion of ESCC cells. CIN85 expression was identified mainly in ESCCs and their adjacent normal tissues, and the high expression of CIN85 was significantly associated with advanced Tumor Node Metastasis stage and lymph node metastasis. CIN85 gene silencing significantly inhibited TE1 cell proliferation, migration and invasion. These results demonstrated that CIN85 was highly expressed in advanced stage ESCC and lymph node metastasis, and played a critical role in tumor proliferation and progression. Therefore, CIN85 may be a promising therapeutic target for human ESCC.
SH3 结构域结合激酶结合蛋白 1（CIN85）是一种 85kDa 的蛋白质，已知是信号衔接家族的成员，在几种人类恶性肿瘤中异常表达，并被发现参与这些肿瘤的生长、迁移和侵袭。本研究的目的是阐明 CIN85 在人食管鳞状细胞癌（ESCC）中的临床意义及其体外功能。采用免疫组织化学法检测 129 例 ESCC 及其相邻正常组织中 CIN85 的表达，探讨其临床相关性和预后价值。采用干扰短发夹 RNA 沉默技术分析 CIN85 在 ESCC TE1 细胞系中的体外功能。采用 MTS、划痕愈合、克隆形成和 Transwell 检测 ESCC 细胞的增殖、迁移和侵袭。CIN85 的表达主要在 ESCC 及其相邻的正常组织中被鉴定，CIN85 的高表达与晚期肿瘤淋巴结转移分期和淋巴结转移显著相关。CIN85 基因沉默显著抑制了 TE1 细胞的增殖、迁移和侵袭。这些结果表明，CIN85 在晚期 ESCC 和淋巴结转移中高表达，在肿瘤增殖和进展中发挥关键作用。因此，CIN85 可能是人类 ESCC 的一个有前途的治疗靶点。
