肿瘤引流淋巴结中 B 和 T 淋巴细胞上 TNFRs 的表达。

Expression of TNFRs by B and T Lymphocytes in Tumor-Draining Lymph Nodes.

机构信息

Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

出版信息

Methods Mol Biol. 2021;2248:259-269. doi: 10.1007/978-1-0716-1130-2_20.

DOI:10.1007/978-1-0716-1130-2_20

PMID:33185883

Abstract

Tumor necrosis factor alpha (TNF-α) has crucial roles in the induction or inhibition of various biological activities in immune and nonimmune cells. This cytokine mainly exerts its effects via two receptors named TNFR1 (CD120a) and TNFR2 (CD120b). Both B and T cells express TNFRs; however, opposing roles have been reported for TNF-α in the adaptive immunity. Lymph nodes (LNs), as the secondary lymphoid organs, are one of the major places for the formation of immune responses against cancer. In this chapter, we explain the procedure as to how to isolate mononuclear cells from tumor-draining lymph nodes. In addition, we describe the process of surface staining with fluorochrome-conjugated antibodies for the assessment of the TNFRs expression by CD3, CD3CD4, CD3CD8, and CD19 lymphocytes by flow cytometry.

摘要

肿瘤坏死因子-α（TNF-α）在免疫和非免疫细胞中各种生物活性的诱导或抑制中起着关键作用。这种细胞因子主要通过两种受体发挥作用，分别命名为 TNFR1（CD120a）和 TNFR2（CD120b）。B 和 T 细胞都表达 TNFRs；然而，TNF-α在适应性免疫中发挥相反的作用。淋巴结（LN）作为次级淋巴器官，是形成针对癌症的免疫反应的主要场所之一。在本章中，我们解释了如何从肿瘤引流淋巴结中分离单核细胞。此外，我们还描述了通过流式细胞术用荧光素标记的抗体进行表面染色的过程，以评估 CD3、CD3CD4、CD3CD8 和 CD19 淋巴细胞上 TNFRs 的表达。