Department of Chemistry, Nanchang University, Nanchang 330031, China.
Department of Chemistry, Nanchang University, Nanchang 330031, China; Jiangxi Province Key Laboratory of Modern Analytical Science, Nanchang University, Nanchang 330031, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Feb 15;247:119146. doi: 10.1016/j.saa.2020.119146. Epub 2020 Nov 4.
In this paper, a novel fluorescence assay has been constructed for the determination of parathion-methyl (PM) by using 4-amino-3-hydroxy-1-naphthalenesulfonic acid (AHNSA) as probe. MnO nanosheets (MnO NS) could quench the fluorescence of AHNSA, while Mn, the reduction product of MnO NS, has no influence on it, resulting in fluorescence recovery. This is because that MnO NS have oxidized characteristic, and they can react with choline (TCh), which is the product of acetylthiocholine (ATCh) catalyzed by acetylcholinesterase (AChE). In the presence of OPs, the activity of AChE was inhibited, accompanied by the restraint of the redox reaction of MnO NS, therefore the fluorescence of AHNSA was quenched. Under the optimized experimental conditions, a linear range of PM was determined to be 0.4-40 ng/mL (R = 0.997) by the proposed method with the limit of detection for 0.18 ng/mL (S/N = 3). The assay was successfully applied to the determination of PM in lake water, which average recoveries were between 86.5% and 114.4%.
本文构建了一种基于 4-氨基-3-羟基-1-萘磺酸(AHNSA)探针用于测定甲基对硫磷(PM)的新型荧光分析法。MnO 纳米片(MnO NS)可以猝灭 AHNSA 的荧光，而 MnO NS 的还原产物 Mn 则对其没有影响，从而导致荧光恢复。这是因为 MnO NS 具有氧化特性，它们可以与胆碱(TCh)反应，TCh 是乙酰胆碱酯酶(AChE)催化乙酰硫代胆碱(ATCh)的产物。在存在有机磷农药(OPs)的情况下，AChE 的活性受到抑制，伴随着 MnO NS 的氧化还原反应的抑制，因此 AHNSA 的荧光被猝灭。在优化的实验条件下，该方法测定 PM 的线性范围为 0.4-40 ng/mL(R = 0.997)，检出限为 0.18 ng/mL(S/N = 3)。该测定方法成功应用于湖水样品中 PM 的测定，平均回收率在 86.5%至 114.4%之间。
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