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甲型流感病毒 H7 血凝素中的编码碱性氨基酸的密码子插入是通过与靠近 snoRNA 结合位点的热点处的 RNA 发生重组而发生的。

Insertions of codons encoding basic amino acids in H7 hemagglutinins of influenza A viruses occur by recombination with RNA at hotspots near snoRNA binding sites.

机构信息

Department of Viroscience, Erasmus Medical Center, 3000 CA Rotterdam, the Netherlands.

Group Imaging and Bioinformatics, Leiden Institute of Advanced Computer Science (LIACS), Leiden University, 2300 RA Leiden, the Netherlands.

出版信息

RNA. 2021 Feb;27(2):123-132. doi: 10.1261/rna.077495.120. Epub 2020 Nov 13.

DOI:10.1261/rna.077495.120
PMID:33188057
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7812872/
Abstract

The presence of multiple basic amino acids in the protease cleavage site of the hemagglutinin (HA) protein is the main molecular determinant of virulence of highly pathogenic avian influenza (HPAI) viruses. Recombination of HA RNA with other RNA molecules of host or virus origin is a dominant mechanism of multibasic cleavage site (MBCS) acquisition for H7 subtype HA. Using alignments of HA RNA sequences from documented cases of MBCS insertion due to recombination, we show that such recombination with host RNAs is most likely to occur at particular hotspots in ribosomal RNAs (rRNAs), transfer RNAs (tRNAs), and viral RNAs. The locations of these hotspots in highly abundant RNAs indicate that RNA recombination is facilitated by the binding of small nucleolar RNA (snoRNA) near the recombination points.

摘要

多个碱性氨基酸在血凝素（HA）蛋白的蛋白酶切割位点的存在是高致病性禽流感（HPAI）病毒毒力的主要分子决定因素。HA RNA 与宿主或病毒来源的其他 RNA 分子的重组是 H7 亚型 HA 获得多碱性切割位点（MBCS）的主要机制。使用由于重组导致 MBCS 插入的 HA RNA 序列的比对，我们表明这种与宿主 RNA 的重组最有可能发生在核糖体 RNA（rRNA）、转移 RNA（tRNA）和病毒 RNA 中的特定热点。这些高丰度 RNA 中的热点位置表明，RNA 重组是由靠近重组点的小核仁 RNA（snoRNA）的结合所促进的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40a7/7812872/5225cd46030e/123f04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40a7/7812872/473f167481a0/123f01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40a7/7812872/2c3bcf90c763/123f02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40a7/7812872/e8a6f362cfb3/123f03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40a7/7812872/5225cd46030e/123f04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40a7/7812872/473f167481a0/123f01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40a7/7812872/2c3bcf90c763/123f02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40a7/7812872/e8a6f362cfb3/123f03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40a7/7812872/5225cd46030e/123f04.jpg

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