从克瓦拉州选定医疗机构的临床标本中分离的多重耐药铜绿假单胞菌中抗生素耐药基因的检测。

Detection of Antibiotic Resistance Genes Among Multiple Drug Resistant Pseudomonas Aeruginosa Isolated from Clinical Sources in Selected Health Institutions in Kwara State.

机构信息

Department of Medical Microbiology and Parasitology, Ladoke Akintola University of Technology, Osogbo, Nigeria.

Department of Microbiology, Kwara State University, Ilorin, Kwara State, Nigeria.

出版信息

Infect Disord Drug Targets. 2021;21(6):e170721187999. doi: 10.2174/1871526520666201116103625.

DOI:10.2174/1871526520666201116103625

PMID:33200719

Abstract

BACKGROUND

Pseudomonas aeruginosa (P. aeruginosa) is a frequent nosocomial pathogen that causes severe diseases in many clinical and community settings. Strains of P. aeruginosa are associated with increased morbidity, mortality and healthcare costs. The rapid emergence of antimicrobial resistance among these strains is a public health crisis. Moreover, there is a paucity of data on the characterization of P. aeruginosa isolates from human clinical samples in Kwara State.

OBJECTIVES

The objectives of this study are to investigate the occurrence of metallo β-lactamase enzyme, multiple antibiotic resistant P. aeruginosa among clinical samples and detection of antibiotic resistance genes among them.

METHODS

Two hundred and thirty-five samples comprising of 145 males and 90 females human clinical specimens were collected aseptically from five selected health institutions within Kwara State, Nigeria. The samples were cultured immediately using standard microbiological procedures. Multiple drug resistance patterns of the micro-organisms to different antibiotics were determined using the Bauer Kirby disc diffusion technique. Metallo β-lactamase production was determined using E - test strip and the DNA samples of the multiple resistant P. aeruginosa strains were extracted and subjected to Polymerase Chain Reaction (PCR) for resistant genes determination. Data were subjected to descriptive statistics using Statistical Package for Social Sciences (SPSS) software.

RESULTS

A total of 145 isolates were identified for P. aeruginosa from the clinical samples. Thirty were positive for metallo β-lactamase production; 11 (8%) males and 19 (13%) females. Absolute resistance to ceftazidime (100%), gentamicin (100%), ceftriaxone (100%) were observed while low resistance to ciprofloxacin (12.4%), piperacillin (6.9%) and imipenem (6.9%). All isolates were sensitive to colistin. The prevalence of various encoding genes blaVIM, , blaCTX-M and blaTEM were 34.4%, 46.7%, 16.7% and 37.7% respectively.

CONCLUSION

This study has shown that there is a high occurrence of metallo β-lactamase enzyme producing and antibiotic-resistant strains of P. aeruginosa in clinical specimens from the studied area. Necessary measures must, therefore be implemented to stop the problems of this antibiotic resistance.

摘要

背景

铜绿假单胞菌（P. aeruginosa）是一种常见的医院病原体，可在许多临床和社区环境中引起严重疾病。铜绿假单胞菌菌株与发病率、死亡率和医疗保健成本增加有关。这些菌株对抗生素的快速出现耐药性是一个公共卫生危机。此外，关于来自尼日利亚夸拉州人类临床样本的铜绿假单胞菌分离株的特征描述的数据很少。

目的

本研究的目的是调查金属β-内酰胺酶酶、多药耐药铜绿假单胞菌在临床样本中的发生情况，并检测其中的抗生素耐药基因。

方法

从尼日利亚夸拉州的五家选定的医疗机构无菌采集了 235 份 145 名男性和 90 名女性的临床标本。使用标准微生物学程序立即对样本进行培养。使用 Bauer Kirby 圆盘扩散技术确定微生物对不同抗生素的多药耐药模式。使用 E-试验条确定金属β-内酰胺酶的产生，提取多药耐药铜绿假单胞菌菌株的 DNA 样本，并进行聚合酶链反应（PCR）以确定耐药基因。使用社会科学统计软件包（SPSS）软件对数据进行描述性统计。

结果

从临床样本中鉴定出 145 株铜绿假单胞菌。30 株产金属β-内酰胺酶阳性；11 株（8%）为男性，19 株（13%）为女性。对头孢他啶（100%）、庆大霉素（100%）、头孢曲松（100%）绝对耐药，对环丙沙星（12.4%）、哌拉西林（6.9%）和亚胺培南（6.9%）耐药较低。所有分离株均对黏菌素敏感。各种编码基因 blaVIM、blaCTX-M 和 blaTEM 的检出率分别为 34.4%、46.7%、16.7%和 37.7%。