Wei Limin, Zeng Bo, Zhang Siyuan, Li Feng, Kong Fanli, Ran Haixia, Wei Hong-Jiang, Zhao Jiangchao, Li Mingzhou, Li Ying
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.
Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan 528231, China.
Animals (Basel). 2020 Nov 16;10(11):2125. doi: 10.3390/ani10112125.
The gut microbiota coevolve with the host and can be stably transmitted to the offspring. Host genetics plays a crucial role in the composition and abundance of gut microbiota. Inbreeding can cause a decrease of the host's genetic diversity and the heterozygosity. In this study, we used 16S rRNA gene sequencing to compare the differences of gut microbiota between the Diannan small-ear pig and Banna minipig inbred, aiming to understand the impact of inbreeding on the gut microbiota. Three dominant bacteria (, , and ) were steadily enriched in both the Diannan small-ear pig and Banna minipig inbred. After inbreeding, the gut microbiota alpha diversity and some potential probiotics (, , , , etc.) were significantly decreased, while the pathogenic bacteria was significantly increased. In addition, the predicted metagenomic analysis (PICRUSt2) indicated that several amino acid metabolisms (''Valine, leucine, and isoleucine metabolism'', ''Phenylalanine, tyrosine, and tryptophan biosynthesis'', ''Histidine metabolism'') were also markedly decreased after the inbreeding. Altogether our data reveal that host inbreeding altered the composition and the predicted function of the gut microbiome, which provides some data for the gut microbiota during inbreeding.
肠道微生物群与宿主共同进化，并可稳定地传递给后代。宿主基因在肠道微生物群的组成和丰度中起着关键作用。近亲繁殖会导致宿主遗传多样性和杂合性降低。在本研究中，我们使用16S rRNA基因测序来比较滇南小耳猪和版纳微型猪近交系之间肠道微生物群的差异，旨在了解近亲繁殖对肠道微生物群的影响。在滇南小耳猪和版纳微型猪近交系中，三种优势菌（、和）均稳定富集。近亲繁殖后，肠道微生物群的α多样性和一些潜在益生菌（、、、等）显著减少，而病原菌显著增加。此外，预测宏基因组分析（PICRUSt2）表明，近亲繁殖后几种氨基酸代谢（“缬氨酸、亮氨酸和异亮氨酸代谢”、“苯丙氨酸、酪氨酸和色氨酸生物合成”、“组氨酸代谢”）也显著减少。总之，我们的数据表明宿主近亲繁殖改变了肠道微生物群的组成和预测功能，这为近亲繁殖期间的肠道微生物群提供了一些数据。
