依普罗沙坦和利巴韦林抑制蜱传脑炎病毒复制的分子和遗传基础。
Molecular and Genetic Bases of Inhibition of Tick-Borne Encephalitis Virus Replication by Eprosartan and Ribavirin.
机构信息
G. P. Somov Institute of Epidemiology and Microbiology, Vladivostok, Russia.
出版信息
Bull Exp Biol Med. 2020 Nov;170(1):53-57. doi: 10.1007/s10517-020-05003-4. Epub 2020 Nov 22.
The antiviral activity of eprosartan (compound selected in silico) towards highly and low-virulent strains of tick-borne encephalitis virus was compared in vitro with activity of ribavirin. Study of the cytopathogenic activity of the virus on SPEV cells by ELISA, IFAT, and PCR showed similar results: both substances (eprosartan and ribavirin) promoted elimination of tick-borne encephalitis virus. Ribavirin exhibited intracellular inhibition towards both strains: the selectivity index for highly virulent Dal'negorsk strain was 160, for low-virulent Primorye-437 strain - 113. Eprosartan inhibited intracellular replication of Dal'negorsk strain (13.7) and less so that of Primorye-437 strain (2.9). The efficiency of virtual screening of the ligand (eprosartan) was demonstrated for highly virulent, but not low virulent tick-borne encephalitis strain.
在体外，比较了依普罗沙坦（计算机筛选的化合物）对高毒力和低毒力蜱传脑炎病毒株的抗病毒活性与利巴韦林的活性。通过 ELISA、IFAT 和 PCR 研究病毒对 SPEV 细胞的细胞病变活性，得到了相似的结果：两种物质（依普罗沙坦和利巴韦林）均促进了蜱传脑炎病毒的消除。利巴韦林对两种毒株均表现出细胞内抑制作用：高毒力的 Dal'negorsk 株的选择性指数为 160，低毒力的 Primorye-437 株为 113。依普罗沙坦抑制 Dal'negorsk 株的细胞内复制（13.7），对 Primorye-437 株的抑制作用较小（2.9）。针对高毒力、而非低毒力蜱传脑炎株，展示了配体（依普罗沙坦）虚拟筛选的效率。
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