氧气对球形红细菌反应中心及捕光多肽编码mRNA水平的影响。
Effect of oxygen on levels of mRNA coding for reaction-centre and light-harvesting polypeptides of Rhodobacter sphaeroides.
作者信息
Hunter C N, Ashby M K, Coomber S A
机构信息
Department of Pure and Applied Biology, Imperial College, London, U.K.
出版信息
Biochem J. 1987 Oct 15;247(2):489-92. doi: 10.1042/bj2470489.
The relative levels of mRNA for the reaction-centre L and M subunits, B875 (LH1) alpha and beta polypeptides and B800-850 (LH2) alpha and beta polypeptides, have been measured during pigment induction of Rhodobacter sphaeroides. Over the 6 h of the experiment, bacteriochlorophyll levels increased by at least 100-fold. No transcripts for photosynthetic components were detectable at the start of induction; after 2 h the levels of transcripts from the puf operon (encoding reaction-centre and B875 subunits) had reached the maximum; these transcripts were 2.7 and 0.5 kb respectively. The transcript for the puc operon (B800-850 complex) was estimated to be 0.55 kb and reached a maximum level after 6 h. These results are consistent with the proposal that, during the assembly of the photosynthetic apparatus, the synthesis of B875 reaction-centre aggregates precedes that of the major antenna, B800-850.
在球形红杆菌色素诱导过程中，已对反应中心L和M亚基、B875（LH1）α和β多肽以及B800 - 850（LH2）α和β多肽的mRNA相对水平进行了测定。在6小时的实验过程中，细菌叶绿素水平增加了至少100倍。诱导开始时未检测到光合组件的转录本；2小时后，来自puf操纵子（编码反应中心和B875亚基）的转录本水平达到最大值；这些转录本分别为2.7 kb和0.5 kb。puc操纵子（B800 - 850复合体）的转录本估计为0.55 kb，并在6小时后达到最大水平。这些结果与以下提议一致，即在光合装置组装过程中，B875反应中心聚集体的合成先于主要天线B800 - 850的合成。
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