College of Chemistry and Life Sciences, Suzhou University of Science and Technology, Suzhou, PR China.
Prep Biochem Biotechnol. 2021;51(7):642-649. doi: 10.1080/10826068.2020.1848868. Epub 2020 Nov 23.
Azo dyes constitute a significant environmental burden due to its toxicity, carcinogenicity, and hard biodegradation. The report here is focused on the decolorization and degradation treatment of azo dye methyl red (MR). Decolorization of MR using LH1 isolated from activated sludge was investigated. The maximum decolorization rate of 92.3% was obtained under the optimized conditions of sucrose as carbon source, 5d incubation age, pH 6.0, 140 mg/L initial concentration of MR and 2.5 g/L initial concentration of NaNO. Biodegradation products of MR were investigated using HPLC-MS, FTIR, and GC-MS assays. It was revealed the three bonds of -C-N = in MR aromatic nucleus were disrupted, and benzoic acid was detected. Micronucleus test with L. and L. demonstrated that MCN‰ (micronucleus permillage) of MR metabolites was less than MR solution. These findings provided evidence that LH1 is a candidate for MR degradation in industrial wastewater treatment.
偶氮染料由于其毒性、致癌性和难以生物降解而对环境造成了重大负担。本报告重点介绍了偶氮染料甲基红（MR）的脱色和降解处理。研究了从活性污泥中分离出的 LH1 对偶氮染料甲基红的脱色作用。在蔗糖作为碳源、5d 培养龄、pH 值 6.0、MR 初始浓度为 140mg/L 和 NaNO3 初始浓度为 2.5g/L 的最佳条件下，获得了 92.3%的最大脱色率。使用 HPLC-MS、FTIR 和 GC-MS 分析方法研究了 MR 的生物降解产物。结果表明，MR 芳核中的 -C-N=N- 三个键被打断，并检测到苯甲酸。用 L. 和 L. 进行微核试验表明，MR 代谢物的 MCN‰（微核百分比）小于 MR 溶液。这些发现为 LH1 是工业废水中 MR 降解的候选物提供了证据。
