Department of Clinical Laboratory, Peking University Shougang Hospital, Beijing, 100144, China.
Department of Pathogen Biology, School of Basic Medicine and Lifescience, Hainan Medical University, Haikou, 571101, China.
BMC Genomics. 2020 Nov 23;21(1):826. doi: 10.1186/s12864-020-07245-y.
Helicobacter himalayensis was isolated from Marmota himalayana in the Qinghai-Tibet Plateau, China, and is a new non-H. pylori species, with unclear taxonomy, phylogeny, and pathogenicity.
A comparative genomic analysis was performed between the H. himalayensis type strain 80(YS1) and other the genomes of Helicobacter species present in the National Center for Biotechnology Information (NCBI) database to explore the molecular evolution and potential pathogenicity of H. himalayensis. H. himalayensis 80(YS1) formed a clade with H. cinaedi and H. hepaticus that was phylogenetically distant from H. pylori. The H. himalayensis genome showed extensive collinearity with H. hepaticus and H. cinaedi. However, it also revealed a low degree of genome collinearity with H. pylori. The genome of 80(YS1) comprised 1,829,936 bp, with a 39.89% GC content, a predicted genomic island, and 1769 genes. Comparatively, H. himalayensis has more genes for functions in "cell wall/membrane/envelope biogenesis" and "coenzyme transport and metabolism" sub-branches than the other compared helicobacters, and its genome contained 42 virulence factors genes, including that encoding cytolethal distending toxin (CDT).
We characterized the H. himalayensis 80(YS1) genome, its phylogenetic position, and its potential pathogenicity. However, further understanding of the pathogenesis of this potentially pathogenic bacterium is required, which might help to manage H. himalayensis-induced diseases.
从中国青藏高原的喜马拉雅旱獭中分离出的喜马拉雅弯曲杆菌是一种新的非幽门螺杆菌物种，其分类学、系统发育和致病性尚不清楚。
对 H. himalayensis 80(YS1) 与 NCBI 数据库中其他弯曲杆菌种的基因组进行了比较基因组分析，以探讨 H. himalayensis 的分子进化和潜在致病性。H. himalayensis 80(YS1)与 H. cinaedi 和 H. hepaticus 形成一个分支，在系统发育上与 H. pylori 相距较远。H. himalayensis 基因组与 H. hepaticus 和 H. cinaedi 具有广泛的共线性，但与 H. pylori 的基因组共线性程度较低。80(YS1)基因组包含 1,829,936 bp，GC 含量为 39.89%，预测有一个基因组岛和 1769 个基因。相比之下，H. himalayensis 在“细胞壁/膜/包膜生物发生”和“辅酶运输和代谢”分支的功能相关基因比其他比较弯曲杆菌更多，其基因组包含 42 个毒力因子基因，包括编码细胞毒素扩张毒素 (CDT)的基因。
我们对 H. himalayensis 80(YS1) 基因组、其系统发育位置及其潜在致病性进行了描述。然而，需要进一步了解这种潜在致病性细菌的发病机制，这可能有助于管理由 H. himalayensis 引起的疾病。
