Rastogi M, Pal N L, Sen A B
Division of Parasitology, Central Drug Research Institute, Lucknow.
Folia Parasitol (Praha). 1987;34(4):289-97.
Effect of temperature on the sporogonic cycle of Plasmodium berghei (NK 65) has been studied in vector Anopheles stephensi. To determine the optimum temperature for development of parasite, fed mosquitoes were kept at 16 +/- 1 degree C, 19 +/- 1 degree C and 26 +/- 1 degree C temperature. The temperature 19 +/- 1 degree C was found to be optimum for normal development of parasite within the vector. Sporulated oocysts were observed on the 10th day post feed and salivary glands were loaded with thousands of sporozoites on day 14. Temperature 16 +/- 1 degree C, though did not exert any deleterious effect on the morphology of parasites it delayed the development of sporozoites in the oocysts by 5 days as compared to control group. In the case of long exposure to this temperature, the sporozoites obtained from salivary glands became non-infective. Temperature 26 +/- 1 degree C was responsible for deterioration of all the stages of sporogonic cycle and it also checked the formation of sporozoites in the oocysts.
在媒介斯氏按蚊中研究了温度对伯氏疟原虫（NK 65）孢子生殖周期的影响。为确定寄生虫发育的最适温度，将吸食血液后的蚊子分别置于16±1℃、19±1℃和26±1℃的温度环境中。结果发现，19±1℃是寄生虫在媒介体内正常发育的最适温度。喂食后第10天观察到有孢子化卵囊，第14天唾液腺中充满了数千个子孢子。16±1℃的温度虽然对寄生虫的形态没有任何有害影响，但与对照组相比，它使卵囊中子孢子的发育延迟了5天。在长时间暴露于该温度的情况下，从唾液腺获得的子孢子变得无感染性。26±1℃导致孢子生殖周期的所有阶段恶化，并且还抑制了卵囊中子孢子的形成。
