Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, La Jolla, California 92093, United States.
Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, 6-3 Aramaki-Aza-Aoba, Aoba-ku, Sendai, Miyagi 980-8578, Japan.
ACS Chem Biol. 2020 Dec 18;15(12):3253-3261. doi: 10.1021/acschembio.0c00824. Epub 2020 Nov 24.
Bacterial hormones, such as the iconic gamma-butyrolactone A-factor, are essential signaling molecules that regulate diverse physiological processes, including specialized metabolism. These low molecular weight compounds are common in species and display species-specific structural differences. Recently, unusual gamma-butyrolactone natural products called salinipostins were isolated from the marine actinomycete genus based on their antimalarial properties. As the salinipostins possess a rare phosphotriester motif of unknown biosynthetic origin, we set out to explore its construction by the widely conserved 9-gene operon in species. We show through a series of and studies that the gene cluster dually encodes the salinipostins and newly identified natural A-factor-like gamma-butyrolactones (Sal-GBLs). Remarkably, homologous biosynthetic gene clusters are widely distributed among many actinomycete genera, including , suggesting the significance of this operon in bacteria.
细菌激素，如标志性的γ-丁内酯 A 因子，是调节包括特殊代谢在内的多种生理过程的必需信号分子。这些低分子量化合物在 物种中很常见，并且显示出物种特异性的结构差异。最近，从海洋放线菌属中分离出了一种不寻常的γ-丁内酯天然产物，称为盐生菌素，这是基于它们的抗疟特性。由于盐生菌素具有未知生物合成来源的罕见膦三酯基序，我们着手探索其在 种中的广泛保守的 9 基因 操纵子中的构建。我们通过一系列的 和 研究表明，该基因簇双重编码盐生菌素和新鉴定的天然 A 因子样γ-丁内酯（Sal-GBLs）。值得注意的是，同源生物合成基因簇广泛分布于许多放线菌属中，包括 ，这表明该操纵子在细菌中的重要性。
