Frank H A, Taremi S S, Knox J R
Department of Chemistry, University of Connecticut, Storrs 06268.
J Mol Biol. 1987 Nov 5;198(1):139-41. doi: 10.1016/0022-2836(87)90466-9.
The photochemical reaction center from Rhodobacter sphaeroides 2.4.1 has been crystallized. The crystals were obtained in a solution of beta-octylglucoside by the vapor diffusion technique using polyethylene glycol 4000 as the precipitant at 22 degrees C. The orthorhombic crystals (space group P2(1)2(1)2(1)) have cell constants a = 142.5 A, b = 136.1 A, c = 78.5 A, and diffract to 3.7 A. The crystals display pronounced linear dichroism in the carotenoid absorption spectral region.
球形红杆菌2.4.1的光化学反应中心已被结晶。通过气相扩散技术,以聚乙二醇4000作为沉淀剂,在β-辛基葡糖苷溶液中于22℃获得晶体。正交晶体(空间群P2(1)2(1)2(1))的晶胞常数为a = 142.5 Å,b = 136.1 Å,c = 78.5 Å,衍射极限为3.7 Å。这些晶体在类胡萝卜素吸收光谱区域表现出明显的线性二色性。