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三维支架平台上分离鼻咽部上皮细胞和癌细胞。

Separation of nasopharyngeal epithelial cells from carcinoma cells on 3D scaffold platforms.

机构信息

Department of Electrical Engineering, City University of Hong Kong, Hong Kong.

Centre for Biosystems, Neuroscience, and Nanotechnology, City University of Hong Kong, Hong Kong.

出版信息

Biotechnol Bioeng. 2021 Apr;118(4):1444-1455. doi: 10.1002/bit.27640. Epub 2020 Dec 13.

Abstract

Scaffold microstructures were developed to mimic a three-dimensional extracellular matrix in studying cell migration and invasion. The multiple-layer scaffold platforms were designed to investigate cell migration and separation from top to bottom layer. Two cell lines including immortalized nasopharyngeal epithelial (NP460) cells and nasopharyngeal carcinoma (NPC43) cells with Epstein-Barr virus were compared in this study. On one-layer platforms with trench depth of 15 µm, both NP460 and NPC43 cells were guided to migrate along the 18-µm-wide trenches, and exhibited random migration directions when the trench width was 10 or 50 µm. Nearly no cell was found to migrate in the 10-µm-wide trenches on one-layer platforms. However, the NP460 and NPC43 cells showed very different probability in the narrow trenches on two-layer platforms, making it possible to separate the nasopharyngeal epithelial cells from the carcinoma cells. Moreover, 1-µm deep grating topography on the top layer inhibited NP460 cells to migrate from top ridges to the 10-µm-wide trenches, but promoted such behavior for NPC43 cells. The results demonstrated in This study suggest that the engineered multiple-layer scaffold platforms could be used to separate carcinoma cells in NPC tumor as a potential treatment of NPC.

摘要

支架微结构的开发是为了模拟三维细胞外基质,以研究细胞迁移和侵袭。多层支架平台的设计目的是从上到下研究细胞迁移和分离。本研究比较了两种细胞系,包括永生化鼻咽上皮(NP460)细胞和携带 EBV 的鼻咽癌细胞(NPC43)。在具有 15μm 深沟槽的单层平台上,NP460 和 NPC43 细胞都被引导沿着 18μm 宽的沟槽迁移,当沟槽宽度为 10μm 或 50μm 时,它们表现出随机的迁移方向。在单层平台的 10μm 宽沟槽中几乎没有细胞迁移。然而,NP460 和 NPC43 细胞在双层平台的狭窄沟槽中表现出非常不同的迁移概率,这使得从鼻咽癌细胞中分离出鼻咽上皮细胞成为可能。此外,顶层 1μm 深的光栅形貌抑制 NP460 细胞从顶层脊迁移到 10μm 宽的沟槽,但促进 NPC43 细胞的这种行为。本研究的结果表明,工程化的多层支架平台可用于分离 NPC 肿瘤中的癌细胞,作为 NPC 的潜在治疗方法。

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