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通过 C. Tropicalis MTCC 6192 对稻秸进行高效酸催化蒸汽预处理以生产木糖醇的过程放大。

Process scale-up of an efficient acid-catalyzed steam pretreatment of rice straw for xylitol production by C. Tropicalis MTCC 6192.

机构信息

Center of Innovative and Applied Bioprocessing (CIAB), Sector-81 (Knowledge city), Mohali 140306, India.

Center of Innovative and Applied Bioprocessing (CIAB), Sector-81 (Knowledge city), Mohali 140306, India.

出版信息

Bioresour Technol. 2021 Jan;320(Pt B):124422. doi: 10.1016/j.biortech.2020.124422. Epub 2020 Nov 18.

Abstract

The present study focuses on operational parameters for the efficient acid catalyzed rice straw pretreatment process for xylitol production. 75.77 % xylose yield was attained when the 24 h presoaked rice straw (≤10 mm or ≤ 15 mm) in 1.5 % (v/v) HSO was pretreated in the same reactor at 121 °C for 30 min. Neutralization with barium hydroxide produced insoluble salt and noticeably reduced HMF and furfurals. Xylitol yield of 0.6 g/g of xylose, was achieved by fermenting rice straw hydrolysate medium with C. tropicalis MTCC 6192. This two-step process of production of xylitol from xylose rich hydrolysate is much simpler and produced minimal inhibitors including organic acids such as acetic acid. This process is modified for upscaling at optimized parameters and will simultaneously minimize the pollution problem caused by rice straw and is also promising for commercial scale.

摘要

本研究专注于高效酸催化稻草预处理工艺的操作参数,以用于木糖生产。当在 121°C 下预处理 30 分钟时,在 1.5%(v/v)HSO 中 24 小时浸泡的≤10mm 或≤15mm 的稻草在相同的反应器中进行预处理,可获得 75.77%的木糖得率。用氢氧化钡中和会产生不溶性盐,并显著降低 HMF 和糠醛。用 C. tropicalis MTCC 6192 发酵富含木糖的水解液,木糖得率可达 0.6g/g。从富含木糖的水解液中生产木糖醇的两步法工艺更简单,产生的抑制剂(包括乙酸等有机酸)更少。该工艺在优化参数下进行放大,可同时最大限度地减少稻草造成的污染问题,在商业规模上也很有前景。

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