全血检测比血浆检测更能增强登革热病毒 RNA 的检测：在登革热疫苗试验中的潜在应用。
Use of whole blood over plasma enhances the detection of dengue virus RNA: possible utility in dengue vaccine trials.
机构信息
Dengue and Chikungunya Group, ICMR-National Institute of Virology, Pune, Maharashtra, 411001, India.
出版信息
Arch Virol. 2021 Feb;166(2):587-591. doi: 10.1007/s00705-020-04892-0. Epub 2020 Nov 27.
In the present study, the utility of viral RNA isolated from whole blood over plasma for detection of dengue virus (DENV) was investigated in 80 samples referred for serotyping by DENV serotype-specific one-step real-time RT-PCR. DENV RNA was detected in 71.25% of the whole blood samples compared to 46.25% in the corresponding plasma samples. In secondary infections, DENV RNA was detected in 83.3% of whole blood samples, while it was detected in 40.5% of plasma samples (P = 0.0001). Non-structural protein 1 (NS1) antigen was detected in only 54.8% of the secondary infections. The detection rate of DENV RNA in whole blood is higher than in plasma. We suggest that one-step real-time RT-PCR using RNA from whole blood combined with an NS1 ELISA should be the choice for dengue diagnosis in dengue vaccine trials.
在本研究中，我们研究了从全血中分离的病毒 RNA 相对于血浆在检测登革热病毒 (DENV) 方面的应用，共 80 例样本通过 DENV 血清型特异性一步法实时 RT-PCR 进行血清分型。与血浆样本相比，全血样本中 DENV RNA 的检出率为 71.25%，而血浆样本中的检出率为 46.25%。在二次感染中，全血样本中 DENV RNA 的检出率为 83.3%，而血浆样本中的检出率为 40.5%（P = 0.0001）。只有 54.8%的二次感染患者检测到非结构蛋白 1（NS1）抗原。全血中 DENV RNA 的检出率高于血浆。因此，我们建议在登革热疫苗试验中，使用全血 RNA 进行一步法实时 RT-PCR 结合 NS1 ELISA 应作为登革热诊断的选择。
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