Department of Medical Biotechnology, University of Siena, Siena, Italy; University Infectious Diseases Unit, Siena University Hospital, Siena, Italy; HIV/AIDS Department, National Institute for Infectious Diseases, IRCCS, L. Spallanzani, Rome, Italy.
University Infectious Diseases Unit, Siena University Hospital, Siena, Italy.
Infez Med. 2020 Nov 1;28(4):576-586.
Single nucleotide polymorphisms (SNPs) in the interleukin 28B (IL28B) gene can influence the course of treated and untreated HBV infection. However, the correlation between different IL28B-SNPs and HBVDNA and quantitative HBsAg (qHBsAg) in chronic HBV infection remains to be fully elucidated. Patients with chronic HBV infection were analysed for qHBsAg, HBVDNA, HBV genotype and six IL28B-SNPs (rs12980275, rs8105790, rs8099917, rs7248668, rs12979860, rs10853728). Seventy patients were recruited: 80% Caucasian, 56% genotype D, 44% treated with nucleos(t)ide analogues, 11% cirrhotic, 37% inactive carriers (IC). Median (IQR) qHBsAg and HBVDNA were 3.2 log10 IU/ml (2.2-3.9) and 2.2 log10 IU/ml (0.3-3.3), respectively. Lower levels of qHBsAg were associated in the whole study population with rs12979860 CC vs. CT (p=0.05), rs12980275 AA vs. AG (p=0.04), rs8105790 TT vs. CT (p=0.05) and genotype D vs. A+E (p=0.01). rs8105790 TT was present in 81% of IC vs. 46% non-IC (p=0.005). These data were also confirmed in the untreated patients' subgroup. In multivariate analysis, IL28B-SNP haplogroups were associated with lower qHBsAg: CC/AA at rs12979860/rs12980275 (-0.70 log IU/mL, 95% CI -1.26;-0.14; p=0.01), CC/TT at rs12979860/rs8105790 (-0.78 log IU/mL, 95% CI -1.33;-0.23; p=0.006) and AA/TT at rs12980275/rs8105790 (-0.71 log IU/mL, 95% CI -1.27;-0.17; p=0.01) both in the whole population and in the untreated subgroup. Specific IL28-SNP haplogroups might be associated with lower qHBsAg.
单核苷酸多态性（SNPs）在白细胞介素 28B（IL28B）基因中可以影响治疗和未治疗的乙型肝炎病毒（HBV）感染的病程。然而，不同的 IL28B-SNPs 与慢性 HBV 感染中的 HBVDNA 和定量 HBsAg（qHBsAg）之间的相关性仍有待充分阐明。分析了慢性 HBV 感染患者的 qHBsAg、HBVDNA、HBV 基因型和六个 IL28B-SNPs（rs12980275、rs8105790、rs8099917、rs7248668、rs12979860、rs10853728）。共招募了 70 名患者：80%为白种人，56%为基因型 D，44%接受核苷（酸）类似物治疗，11%为肝硬化，37%为非活动携带者（IC）。qHBsAg 和 HBVDNA 的中位数（IQR）分别为 3.2 log10 IU/ml（2.2-3.9）和 2.2 log10 IU/ml（0.3-3.3）。在整个研究人群中，qHBsAg 水平较低与 rs12979860 CC 比 CT（p=0.05）、rs12980275 AA 比 AG（p=0.04）、rs8105790 TT 比 CT（p=0.05）和基因型 D 比 A+E（p=0.01）相关。rs8105790 TT 存在于 81%的 IC 中，而非 IC 中为 46%（p=0.005）。这些数据在未治疗患者亚组中也得到了证实。在多变量分析中，IL28B-SNP 单倍型与较低的 qHBsAg 相关：rs12979860/rs12980275 处的 CC/AA（-0.70 log IU/mL，95%CI -1.26；-0.14；p=0.01）、rs12979860/rs8105790 处的 CC/TT（-0.78 log IU/mL，95%CI -1.33；-0.23；p=0.006）和 rs12980275/rs8105790 处的 AA/TT（-0.71 log IU/mL，95%CI -1.27；-0.17；p=0.01），无论是在整个人群还是未治疗亚组中均如此。特定的 IL28-SNP 单倍型可能与较低的 qHBsAg 相关。
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