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Katanin p60 样蛋白 1 塑造机械敏感纤毛中的细胞骨架。

Katanin p60-like 1 sculpts the cytoskeleton in mechanosensory cilia.

机构信息

Tsinghua-Peking Joint Center for Life Science, School of Life Sciences, Tsinghua University, Beijing, China.

School of Medicine, Tsinghua University, Beijing, China.

出版信息

J Cell Biol. 2021 Jan 4;220(1). doi: 10.1083/jcb.202004184.

DOI:10.1083/jcb.202004184
PMID:33263729
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7717695/
Abstract

Mechanoreceptor cells develop a specialized cytoskeleton that plays structural and sensory roles at the site of mechanotransduction. However, little is known about how the cytoskeleton is organized and formed. Using electron tomography and live-cell imaging, we resolve the 3D structure and dynamics of the microtubule-based cytoskeleton in fly campaniform mechanosensory cilia. Investigating the formation of the cytoskeleton, we find that katanin p60-like 1 (kat-60L1), a neuronal type of microtubule-severing enzyme, serves two functions. First, it amplifies the mass of microtubules to form the dense microtubule arrays inside the sensory cilia. Second, it generates short microtubules that are required to build the nanoscopic cytoskeleton at the mechanotransduction site. Additional analyses further reveal the functional roles of Patronin and other potential factors in the local regulatory network. In all, our results characterize the specialized cytoskeleton in fly external mechanosensory cilia at near-molecular resolution and provide mechanistic insights into how it is formed.

摘要

机械感受器细胞发育出一种特殊的细胞骨架，在机械转导部位发挥结构和感觉作用。然而，对于细胞骨架的组织和形成方式，我们知之甚少。本研究利用电子断层扫描和活细胞成像技术，解析了果蝇机械敏感毛状细胞中基于微管的细胞骨架的三维结构和动态。在研究细胞骨架的形成过程中，我们发现 katanin p60-like 1（kat-60L1），一种神经元型的微管切割酶，具有双重功能。首先，它放大微管的质量，在感觉纤毛内形成密集的微管阵列。其次，它产生短的微管，这些微管对于在机械转导部位构建纳米级细胞骨架是必需的。进一步的分析还揭示了 Patronin 和其他潜在因素在局部调控网络中的功能作用。总之，我们的结果以近分子分辨率描绘了果蝇外部机械感受器纤毛中的特化细胞骨架，并为其形成方式提供了机制见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbd9/7717695/5203287f7ba6/JCB_202004184_Fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbd9/7717695/16079f7804e8/JCB_202004184_Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbd9/7717695/f7b0611f2d6a/JCB_202004184_Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbd9/7717695/8080ea4df9d2/JCB_202004184_FigS1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbd9/7717695/594a7637c338/JCB_202004184_FigS2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbd9/7717695/069d0da26293/JCB_202004184_Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbd9/7717695/5bbc9ffe616b/JCB_202004184_Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbd9/7717695/b4f3109ceed5/JCB_202004184_FigS3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbd9/7717695/9af0338c9194/JCB_202004184_Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbd9/7717695/99f797f9f0b9/JCB_202004184_FigS4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbd9/7717695/003803fa4269/JCB_202004184_FigS5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbd9/7717695/0bbdfe4117d7/JCB_202004184_Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbd9/7717695/c178b6760139/JCB_202004184_Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbd9/7717695/5203287f7ba6/JCB_202004184_Fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbd9/7717695/16079f7804e8/JCB_202004184_Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbd9/7717695/f7b0611f2d6a/JCB_202004184_Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbd9/7717695/8080ea4df9d2/JCB_202004184_FigS1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbd9/7717695/594a7637c338/JCB_202004184_FigS2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbd9/7717695/069d0da26293/JCB_202004184_Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbd9/7717695/5bbc9ffe616b/JCB_202004184_Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbd9/7717695/b4f3109ceed5/JCB_202004184_FigS3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbd9/7717695/9af0338c9194/JCB_202004184_Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbd9/7717695/99f797f9f0b9/JCB_202004184_FigS4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbd9/7717695/003803fa4269/JCB_202004184_FigS5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbd9/7717695/0bbdfe4117d7/JCB_202004184_Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbd9/7717695/c178b6760139/JCB_202004184_Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbd9/7717695/5203287f7ba6/JCB_202004184_Fig8.jpg

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