通过凝胶和印迹覆盖法对高分子量蛋白质进行免疫检测。

Immunological detection of high molecular weight proteins by gel and blot overlay.

作者信息

Riederer B M, Goodman S R

机构信息

Department of Physiology, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey 17033.

出版信息

Brain Res Bull. 1987 Dec;19(6):715-22. doi: 10.1016/0361-9230(87)90059-1.

DOI:10.1016/0361-9230(87)90059-1

PMID:3326648

Abstract

The sensitivity and specificity of the gel overlay and western blot methods of immunodetection are compared for spectrins, typical high molecular weight proteins. The gel overlay method is more sensitive and specific for the immunodetection of brain spectrin (240/235) and rbc spectrin. As the western blot technique will remain the method of choice for many applications because of its relative speed, we discuss methods for optimizing its sensitivity and selectivity.

摘要

针对血影蛋白（一种典型的高分子量蛋白质），比较了凝胶覆盖法和蛋白质免疫印迹法这两种免疫检测方法的灵敏度和特异性。凝胶覆盖法在检测脑血影蛋白（240/235）和红细胞血影蛋白方面更灵敏且更具特异性。由于蛋白质免疫印迹技术相对快速，在许多应用中仍将是首选方法，因此我们讨论了优化其灵敏度和选择性的方法。