Duanmu Cheng-Lin, Wang Xiao-Yu, Zhang Xiao-Ning, He Wei, Su Yang-Shuai, Wan Hong-Ye, Hu Ling, Wang Yi, Jing Xiang-Hong
Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhen Ci Yan Jiu. 2020 Nov 25;45(11):902-7. doi: 10.13702/j.1000-0607.200155.
To observe and compare the ameliorate effects of electroacupuncture (EA) and transcutaneous electrical acupoint stimulation (TEAS) with different intensities on inflammatory muscle pain, so as to confirm the role of different afferent nerve fibers in different layers (skin or muscle) in pain relief of acupuncture.
The intensities of the thresholds of A (Ta) and C (Tc) were selected for TEAS and EA. TEAS and EA were applied on the layer of skin and muscle of "Liangqiu" (ST34), respectively. Forty-eight rats were randomly divided into control, model, TEAS-Ta, TEAS-Tc, EA-Ta and EA-Tc groups (=8 in each group). The inflammatory muscle pain model was established by complete Freund's adjuvant (CFA) injection into the right biceps femoris muscle. EA or TEAS was applied once a day for 3 days. The weight difference between the hind limbs and the abnormal electromyography (EMG) activities were observed as an index for pain of the rats.
After modeling, the weight difference between the hind limbs was increased markedly in the model group relevant to the control group (<0.01). Compared with the model group, the weight difference between the hind limbs in the TEAS-Tc, EA-Ta, and EA-Tc groups was significantly decreased (<0.05, <0.01). Compared with that before intervention, the area under the curve and discharge frequency of abnormal EMG of rats in the TEAS-Tc and EA-Ta groups were significantly reduced after intervention (<0.01, <0.05), while those in the EA-Tc group were significantly increased (<0.05). After the intervention, compared with the TEAS-Ta group, the inhibition rate of the area under the curve and the discharge frequency of the abnormal EMG in the TEAS-Tc group increased significantly (P＜0.05); and those were also increased in the EA-Ta group when compared with the EA-Tc group (<0.01).
TEAS with Tc or EA with Ta in the muscle layer can alleviate the pain and inhibit the abnormal EMG in inflammatory muscle pain rats. It is indicated that local pain relief by acupuncture was related to the afferent nerve fiber in different layer with different intensities.
观察并比较不同强度的电针（EA）和经皮穴位电刺激（TEAS）对炎性肌肉疼痛的改善作用，以明确不同层次（皮肤或肌肉）的不同传入神经纤维在针刺镇痛中的作用。
选取A（Ta）和C（Tc）阈值强度用于TEAS和EA。TEAS和EA分别作用于“梁丘”（ST34）的皮肤层和肌肉层。48只大鼠随机分为对照组、模型组、TEAS-Ta组、TEAS-Tc组、EA-Ta组和EA-Tc组（每组8只）。通过向右侧股二头肌注射完全弗氏佐剂（CFA）建立炎性肌肉疼痛模型。EA或TEAS每天施加1次，共3天。观察大鼠后肢重量差异和异常肌电图（EMG）活动作为大鼠疼痛指标。
建模后，模型组后肢重量差异与对照组相比明显增加（P<0.01）。与模型组相比，TEAS-Tc组、EA-Ta组和EA-Tc组后肢重量差异明显降低（P<0.05，P<0.01）。与干预前相比，TEAS-Tc组和EA-Ta组大鼠干预后异常EMG的曲线下面积和放电频率明显降低（P<0.01，P<0.05），而EA-Tc组明显增加（P<0.05）。干预后，与TEAS-Ta组相比，TEAS-Tc组异常EMG曲线下面积和放电频率的抑制率明显增加（P＜0.05）；与EA-Tc组相比，EA-Ta组也增加（P<0.01）。
肌肉层采用Tc强度的TEAS或Ta强度的EA可减轻炎性肌肉疼痛大鼠的疼痛并抑制异常EMG。表明针刺局部镇痛与不同强度的不同层次传入神经纤维有关。
