Research Faculty of Agriculture, Hokkaido University.
Microbes Environ. 2020;35(4). doi: 10.1264/jsme2.ME20100.
A nitrous oxide (NO)-consuming bacterium isolated from farmland soil actively consumed NO under high pH conditions. An acetylene inhibition assay did not show the denitrification of N to NO by this bacterium. When NO was injected as the only nitrogen source, this bacterium did not assimilate NO. A polymerase chain reaction demonstrated that this bacterium did not have the typical nosZ gene. This bacterium belonged to Chitinophagaceae, but did not belong to known families that include bacteria with the atypical nosZ. This is the first study to show that a non-denitrifier actively reduces NO, even under high pH conditions.
从农田土壤中分离到的一种消耗一氧化二氮（NO）的细菌在高 pH 值条件下能主动消耗 NO。乙炔抑制试验表明，该细菌不会将 N 反硝化为 NO。当将 NO 作为唯一氮源注入时，该细菌不会同化 NO。聚合酶链反应表明，该细菌没有典型的 nosZ 基因。该细菌属于噬几丁质菌科，但不属于包括具有非典型 nosZ 的细菌在内的已知科。这是首次表明非反硝化菌能在高 pH 值条件下主动还原 NO 的研究。
