昼夜节律脂质组学：分析 24 小时内的脂质代谢物。

Circadian Lipidomics: Analysis of Lipid Metabolites Around the Clock.

机构信息

Division of Endocrinology, Diabetes and Nutrition Division, Department of Medicine, Faculty of Medicine, University of Geneva, Geneva, Switzerland.

Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Geneva, Switzerland.

出版信息

Methods Mol Biol. 2021;2130:169-183. doi: 10.1007/978-1-0716-0381-9_13.

DOI:10.1007/978-1-0716-0381-9_13

PMID:33284444

Abstract

Lipidomics has been defined as the large-scale analysis of lipids in organelles, cells, tissues, or whole organisms. Including the temporal aspects of lipid metabolic changes into this analysis allows to access yet another important aspect of lipid regulation. The resulting methodology, circadian lipidomics, has thus emerged as a novel tool to address the enormous complexity, which is present among cellular lipids. Here, we describe how mass spectrometry-based circadian lipidomics can be applied to study the impact of peripheral clocks on lipid metabolism in human primary cells and tissues, exemplified by studies in human pancreatic islets and skeletal myotubes.

摘要

脂质组学被定义为细胞器、细胞、组织或整个生物体中脂质的大规模分析。将脂质代谢变化的时间方面纳入到这种分析中，可以获得脂质调节的另一个重要方面。由此产生的方法，即昼夜节律脂质组学，因此成为解决细胞脂质中存在的巨大复杂性的一种新工具。在这里，我们描述了如何应用基于质谱的昼夜节律脂质组学来研究外周时钟对人原代细胞和组织中脂质代谢的影响，以人胰岛和骨骼肌成肌细胞为例。