Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou, China.
Pest Manag Sci. 2021 Apr;77(4):1873-1885. doi: 10.1002/ps.6212. Epub 2021 Jan 5.
Azadirachtin has the potential to be used for pest control. Nevertheless, few studies have investigated the effects of azadirachtin on the cognitive behavior of pests. In this study, expression of the cAMP response element-binding protein (CREB) and its gene were studied via a series of experiments in Spodoptera litura larvae treated with azadirachtin.
RNA-Seq analysis of S. litura larvae treated with azadirachtin was undertaken. According to Kyoto Encyclopedia of Genes and Genomes analysis, the top 20 enriched pathways included neuroactive ligand-receptor interaction pathways, with seven significantly differentially expressed genes including CREB. Quantitative real time polymerase chain reaction (qRT-PCR) results indicated that the CREB gene was expressed during all developmental stages of S. litura, but relative expression of the CREB gene was significantly downregulated after treatment with azadirachtin. Grayscale statistical analysis also showed that expression levels of protein kinase A (PKA), extracellular signal-regulated kinase (ERK) and CREB proteins were significantly downregulated after treatment with azadirachtin. Moreover, RNA interference results showed that the effect of azadirachtin on the cognitive behavior of S. litura was consistent with that seen after interfering with CREB. In addition, larval selectivity to addictive odor sources was reduced, and the initial reaction time was increased.
This study clarified that azadirachtin can affect the cognitive behavior of S. litura and treatment with azadirachtin resulted in a downregulation of gene and protein expression of CREB and its pathway proteins. © 2020 Society of Chemical Industry.
印楝素具有作为害虫防治剂的潜力。然而,很少有研究调查印楝素对害虫认知行为的影响。在这项研究中,通过一系列实验研究了印楝素处理的斜纹夜蛾幼虫中环磷酸腺苷反应元件结合蛋白(CREB)及其基因的表达。
对印楝素处理的斜纹夜蛾幼虫进行了 RNA-Seq 分析。根据京都基因与基因组百科全书分析,前 20 个富集途径包括神经活性配体-受体相互作用途径,其中有 7 个显著差异表达的基因包括 CREB。实时定量聚合酶链反应(qRT-PCR)结果表明,CREB 基因在斜纹夜蛾的所有发育阶段都有表达,但在印楝素处理后,CREB 基因的相对表达水平显著下调。灰度统计分析也表明,蛋白激酶 A(PKA)、细胞外信号调节激酶(ERK)和 CREB 蛋白的表达水平在印楝素处理后显著下调。此外,RNA 干扰结果表明,印楝素对斜纹夜蛾认知行为的影响与干扰 CREB 后的影响一致。此外,幼虫对成瘾性气味源的选择性降低,初始反应时间延长。
本研究阐明了印楝素可以影响斜纹夜蛾的认知行为,并且印楝素处理导致 CREB 及其途径蛋白的基因和蛋白表达下调。 © 2020 化学工业协会。