Seo Seung-Jun, Kim Yong-Gun
Department of Periodontology, School of Dentistry, A3DI, Kyungpook National University, Daegu, Republic of Korea.
Institute for Translational Research in Dentistry, Kyungpook National University, Daegu, Republic of Korea.
J Appl Biomater Funct Mater. 2020 Jan-Dec;18:2280800020963476. doi: 10.1177/2280800020963476.
The conventional micro-computed tomography (μCT) is a non-destructive imaging technique used for obtaining 2D and 3D information for scaffolds. The main composition and internal structure are important in mimicking and designing the characteristics of natural bone. This study was three-dimensional evaluating the external or internal structures and the hydration effects of bone graft materials by using the in-situ image technique. Synchrotron radiation micro-computed tomography (SR-μCT) was used to extract information on the geometry of two biphasic calcium phosphates (BCP) with identical chemicals and different micro-macro porosity, pore size distribution, and pore interconnection pathways. Volume analysis by hydration was used to measure the two bone graft materials at 0, 5, and 10-min intervals. The SR-μCT image was achieved with information regarding the internal pore structure and hydration effects evaluated under 3D visualization. Both types of bone graft materials showed structures suitable for tissue engineering applications. The SR-μCT in-situ techniques with 3D information provided a detailed view of the structures. Thus, SR-μCT could be an available, unbiased 3D alternative to in-situ analysis.
传统的微型计算机断层扫描（μCT）是一种非破坏性成像技术，用于获取支架的二维和三维信息。支架的主要组成和内部结构对于模拟和设计天然骨的特性至关重要。本研究通过使用原位成像技术对骨移植材料的外部或内部结构以及水化作用进行三维评估。同步辐射微型计算机断层扫描（SR-μCT）用于提取两种具有相同化学成分但微观-宏观孔隙率、孔径分布和孔隙互连途径不同的双相磷酸钙（BCP）的几何信息。通过水化进行体积分析，以0、5和10分钟的间隔测量两种骨移植材料。通过三维可视化评估内部孔隙结构和水化作用的信息，获得了SR-μCT图像。两种类型的骨移植材料均显示出适合组织工程应用的结构。具有三维信息的SR-μCT原位技术提供了结构的详细视图。因此，SR-μCT可以成为一种可用的、无偏差的三维原位分析替代方法。
