College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, P. R. China.
Chem Biodivers. 2021 Jan;18(1):e2000710. doi: 10.1002/cbdv.202000710. Epub 2020 Dec 22.
In this study, the pine nut (Pinus yunnanensis Franch.) protein was hydrolyzed by alkaline protease and trypsin to prepare pine nut protein hydrolysate (PNPH). The chemical, intracellular and in vivo antioxidant capacity of PNPH were evaluated. PNPH owned the ability of scavenging free radicals, and it could protect the HepG2 cells from oxidative damage by preserving cell viability. Moreover, PNPH could reduce the malondialdehyde (MDA) content and improved the superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in serum, heart and liver of aging mice induced by D-galactose. Further, the PNPH was stepwise purified and identified, and 15 peptides were identified from purified fraction in PNPH. The three-dimension structures of identified peptides were predicted. Among all identified peptides, peptide 3, 7, 8 and 11 were presumed to possess good antioxidant activity. Overall, PNPH and purified peptides isolated from PNPH have potential application prospects in the field of natural antioxidants and anti-aging functional foods.
在这项研究中，采用碱性蛋白酶和胰蛋白酶水解云南松（Pinus yunnanensis Franch.）蛋白，制备云南松蛋白水解物（PNPH）。评价了 PNPH 的化学性质、细胞内和体内抗氧化能力。PNPH 具有清除自由基的能力，可通过保持细胞活力来保护 HepG2 细胞免受氧化损伤。此外，PNPH 可降低 D-半乳糖诱导衰老小鼠血清、心脏和肝脏中丙二醛（MDA）含量，提高超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的活性。进一步对 PNPH 进行分步纯化和鉴定，从 PNPH 中纯化的馏分中鉴定出 15 个肽。预测了鉴定出的肽的三维结构。在所有鉴定出的肽中，推测肽 3、7、8 和 11 具有良好的抗氧化活性。总的来说，PNPH 和从 PNPH 中分离出的纯化肽在天然抗氧化剂和抗衰老功能性食品领域具有潜在的应用前景。
