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USP8基因敲低抑制肺癌细胞生长。

Knockdown of USP8 Inhibits the Growth of Lung Cancer Cells.

作者信息

Rong Zhenhua, Zhu Zongmin, Cai Shihua, Zhang Bingqing

机构信息

Minimally Invasive Surgery Oncology, The People's Hospital of Caoxian, Heze, Shandong, People's Republic of China.

Department of Pharmacology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, People's Republic of China.

出版信息

Cancer Manag Res. 2020 Dec 2;12:12415-12422. doi: 10.2147/IJN.S259191. eCollection 2020.

DOI:10.2147/IJN.S259191
PMID:33293867
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7719325/
Abstract

PURPOSE: Lung cancer is the deadliest tumor in the world. This study aimed to investigate the effection of USP8 on the proliferation and growth of NSCLC cells. METHODS: The proliferation, migration, invasion, cell cycle progression, and apoptosis of A549 and H1299 cells were evaluated with CCK8, colony formation, scratch, transwell, and flow cytometry experiments. Furthermore, the expression of cell cycle- and apoptosis-related proteins was detected by western blot. RESULTS: Knockdown of USP8 inhibited the proliferation, migration, invasion, and cell cycle progression of A549 and H1299 cells, and promoted the apoptosis. The results of western blot indicated that knockdown of USP8 down-regulated the expression of Cyclin D1, CDK4, CDK6, p-AKT, and Bcl2, and up-regulated the expression of Bax. CONCLUSION: Knockdown of USP8 inhibited the proliferation of human lung cancer cells by regulating cell cycle- and apoptosis-related proteins. USP8 may be a therapeutic target for lung cancer.

摘要

目的：肺癌是全球最致命的肿瘤。本研究旨在探讨泛素特异性蛋白酶8（USP8）对非小细胞肺癌（NSCLC）细胞增殖和生长的影响。 方法：通过CCK8、集落形成、划痕、Transwell和流式细胞术实验评估A549和H1299细胞的增殖、迁移、侵袭、细胞周期进程和凋亡。此外，通过蛋白质免疫印迹法检测细胞周期和凋亡相关蛋白的表达。 结果：敲低USP8可抑制A549和H1299细胞的增殖、迁移、侵袭及细胞周期进程，并促进细胞凋亡。蛋白质免疫印迹结果表明，敲低USP8可下调细胞周期蛋白D1（Cyclin D1）、细胞周期蛋白依赖性激酶4（CDK4）、细胞周期蛋白依赖性激酶6（CDK6）、磷酸化蛋白激酶B（p-AKT）和B细胞淋巴瘤/白血病-2（Bcl2）的表达，并上调Bax的表达。 结论：敲低USP8通过调节细胞周期和凋亡相关蛋白抑制人肺癌细胞的增殖。USP8可能是肺癌的一个治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1353/7719325/7950d383ee5a/CMAR-12-12415-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1353/7719325/0a180eef8c5c/CMAR-12-12415-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1353/7719325/328841c6c5e2/CMAR-12-12415-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1353/7719325/38f9d891d2e3/CMAR-12-12415-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1353/7719325/7950d383ee5a/CMAR-12-12415-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1353/7719325/0a180eef8c5c/CMAR-12-12415-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1353/7719325/328841c6c5e2/CMAR-12-12415-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1353/7719325/38f9d891d2e3/CMAR-12-12415-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1353/7719325/7950d383ee5a/CMAR-12-12415-g0004.jpg

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引用本文的文献

[1]
USP8-mediated PTK7 promotes PIK3CB-related pathway to accelerate the malignant progression of non-small cell lung cancer.

Thorac Cancer. 2025-1

[2]
Fusion of Platelet Derived Growth Factor Receptor Alpha () With Ubiquitin Specific Peptidase 8 () in a Calcified Chondroid Mesenchymal Neoplasm Harboring t(4;15)(q12;q21) as a Sole Aberration.

Cancer Genomics Proteomics. 2024

[3]
Establishment of a prognosis predictive model for liver cancer based on expression of genes involved in the ubiquitin-proteasome pathway.

World J Clin Oncol. 2024-3-24

[4]
Targeting USP8 Inhibits O-GlcNAcylation of SLC7A11 to Promote Ferroptosis of Hepatocellular Carcinoma via Stabilization of OGT.

Adv Sci (Weinh). 2023-11

[5]
Ubiquitin-proteasome pathway-mediated regulation of the Bcl-2 family: effects and therapeutic approaches.

Haematologica. 2024-1-1

[6]
USP8 positively regulates hepatocellular carcinoma tumorigenesis and confers ferroptosis resistance through β-catenin stabilization.

Cell Death Dis. 2023-6-13

[7]
Inhibition of ubiquitin specific peptidase 8 is effective against 5-fluorouracil resistance in colon cancer via suppressing EGFR and EGFR-mediated signaling pathways.

Histol Histopathol. 2024-2

[8]
Targeting ubiquitin-specific protease 8 sensitizes anti-programmed death-ligand 1 immunotherapy of pancreatic cancer.

Cell Death Differ. 2023-2

[9]
Ubiquitin specific peptidase 11 as a novel therapeutic target for cancer management.

Cell Death Discov. 2022-6-17

[10]
Molecular mechanism of lncRNA SNHG12 in immune escape of non-small cell lung cancer through the HuR/PD-L1/USP8 axis.

Cell Mol Biol Lett. 2022-6-3

本文引用的文献

[1]
Deubiquitylation and stabilization of Notch1 intracellular domain by ubiquitin-specific protease 8 enhance tumorigenesis in breast cancer.

Cell Death Differ. 2020-4

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J Cell Biochem. 2018-8-21

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Oncogene. 2018-6-7

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J Cancer Prev. 2015-3

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Chest. 2013-5

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Future Oncol. 2011-5

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