缅甸北部地区结核分枝杆菌 24 位基因间隔寡核苷酸重复分型和 spoligotyping 技术的基因多样性研究。

Genetic diversity of Mycobacterium tuberculosis using 24-locus MIRU-VNTR typing and Spoligotyping in Upper Myanmar.

机构信息

Medical Technology Program, Faculty of Associated medical Sciences, Khon Kaen University, Khon Kaen,Thailand.

Department of Microbiology, Faculty of Medicine, Khon Kaen University, Thailand.

出版信息

J Infect Dev Ctries. 2020 Nov 30;14(11):1296-1305. doi: 10.3855/jidc.12998.

DOI:10.3855/jidc.12998

PMID:33296343

Abstract

INTRODUCTION

MIRU-VNTR typing and Spoligotyping are the useful molecular tools for TB epidemiology study. Information regarding genetic diversity and tuberculosis (TB) transmission in Upper Myanmar only is scares.

METHODOLOGY

We determined the genetic diversity of Mycobacterium tuberculosis (Mtb) and TB transmission from Upper Myanmar TB Reference Laboratory, Mandalay Region, including Mandalay (72), Shan (22), Magway (15), Sagaing (13), Nay Pyi Taw (8), Kachin (7), Chin (2) and Kayah (1). One hundred and forty Mtb isolates were genotyped using 24-locus MIRU-VNTR typing and spoligotyping. Lineage classification and TB transmission analysis were performed.

RESULTS

24-locus MIRU-VNTR typing identified 135 unique profiles and two clusters compared to 35 spoligotyping profiles which contained 12 clusters and 23 unique isolates, Beijing (n=100, 71.4%) was found to be prominent lineage by combine two methods. The expected proportion attributable to recent transmission based on clustering rate was 2.1%. One cluster case was more likely to be in MDR patient.

CONCLUSIONS

Our findings showed Beijing genotypes were dominant in Upper Myanmar. The usage and analysis of 24-locus MIRU-VNTR typing might prove useful for our broader understanding of TB outbreaks and epidemiology than spoligotyping. The genotypic pattern of this combined method suggests that the lower transmission rate may be due to a higher possibility of reactivation cases in Upper Myanmar.

摘要

简介

MIRU-VNTR 分型和 spoligotyping 是结核病流行病学研究的有用分子工具。有关上缅甸遗传多样性和结核病（TB）传播的信息很少。

方法

我们从缅甸上缅甸结核病参考实验室（位于曼德勒地区，包括曼德勒[72]、掸邦[22]、马圭[15]、实皆[13]、内比都[8]、克钦邦[7]、钦邦[2]和克伦邦[1]）确定了结核分枝杆菌（Mtb）的遗传多样性和 TB 传播。使用 24 位基因 MIRU-VNTR 分型和 spoligotyping 对 140 株 Mtb 分离株进行了基因分型。进行了谱系分类和 TB 传播分析。

结果

与包含 12 个聚类和 23 个独特分离株的 35 个 spoligotyping 图谱相比，24 位基因 MIRU-VNTR 分型鉴定出 135 个独特图谱和 2 个聚类。通过结合两种方法，发现北京（n=100，71.4%）是主要的谱系。基于聚类率，归因于近期传播的预期比例为 2.1%。一个聚类病例更有可能是耐多药患者。