Zhang Jian, Yuan Huwei, Li Yujuan, Chen Yanhong, Liu Guoyuan, Ye Meixia, Yu Chunmei, Lian Bolin, Zhong Fei, Jiang Yuna, Xu Jichen
Key Lab of Landscape Plant Genetics and Breeding, School of Life Science, Nantong University, 226019, Nantong, China.
State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, 311300, Hangzhou, China.
Hortic Res. 2020 Dec 1;7(1):201. doi: 10.1038/s41438-020-00424-8.
Polyploidy is a common phenomenon among willow species. In this study, genome sequencing was conducted for Salix matsudana Koidz (also named Chinese willow), an important greening and arbor tree species, and the genome of this species was compared with those of four other tree species in Salicaceae. The total genome sequence of S. matsudana was 655.72 Mb in size, with repeated sequences accounting for 45.97% of the total length. In total, 531.43 Mb of the genome sequence could be mapped onto 38 chromosomes using the published genetic map as a reference. The genome of S. matsudana could be divided into two groups, the A and B genomes, through homology analysis with the genome of Populus trichocarpa, and the A and B genomes contained 23,985 and 25,107 genes, respectively. 4DTv combined transposon analysis predicted that allotetraploidy in S. matsudana appeared ~4 million years ago. The results from this study will help reveal the evolutionary history of S. matsudana and lay a genetic basis for its breeding.
多倍体现象在柳树物种中很常见。在本研究中，对重要的绿化乔木树种旱柳（也称为中国柳树）进行了基因组测序，并将该物种的基因组与杨柳科其他四个树种的基因组进行了比较。旱柳的基因组序列总长为655.72 Mb，重复序列占总长度的45.97%。以已发表的遗传图谱为参考，总共531.43 Mb的基因组序列可定位到38条染色体上。通过与毛果杨的基因组进行同源性分析，旱柳的基因组可分为A和B两组，A和B基因组分别包含23,985和25,107个基因。4DTv联合转座子分析预测，旱柳的异源四倍体大约出现在400万年前。本研究结果将有助于揭示旱柳的进化历史，并为其育种奠定遗传基础。
