Johnson A L, Moutray M, Hoffmann W E
Department of Veterinary Clinical Medicine, University of Illinois, Urbana.
Vet Surg. 1987 Nov-Dec;16(6):418-22. doi: 10.1111/j.1532-950x.1987.tb00980.x.
Two hundred seventeen cortical bones were harvested cleanly and prepared for banking for 2 months by using one of three types of packing materials, two ethylene oxide (EO) concentrations and procedures, and two storage temperatures. Bone subjected to the various treatments was compared to freshly harvested cortical bone and bone tested immediately after sterilization. Parameters evaluated were handling characteristics during preparation for and placement of a 3.5 mm cortex screw, and the percentage of weight lost as water when the bones were dried in an oven at 100 degrees C for 72 hours. Methods of sterilization, packaging material, and temperature of storage affected the handling characteristics and dehydration of the bone specimens. Twelve per cent EO at an elevated temperature and pressure, paper packaging, and room temperature storage appeared to cause the most significant changes. The use of 84% EO at room temperature and pressure, polyethylene wrapping material, and storage at -20 degrees C appeared to protect bone from dehydration. There was an increase in cracking and splitting of the bones as the percent of water loss decreased (indicating dehydration at the time of testing). Dehydration due to sterilization and storage processes may lead to difficulty in preparing bone for insertion of a bone screw and subsequently jeopardizing the stability of a repair in which such alloimplants are used.
收集了217块皮质骨并清理干净，使用三种包装材料之一、两种环氧乙烷（EO）浓度和程序以及两种储存温度，将其准备用于储存2个月。将经过各种处理的骨与新鲜采集的皮质骨以及灭菌后立即测试的骨进行比较。评估的参数包括准备和放置3.5毫米皮质螺钉时的操作特性，以及将骨在100摄氏度烤箱中干燥72小时后作为水分损失的重量百分比。灭菌方法、包装材料和储存温度影响了骨标本的操作特性和脱水情况。在高温高压下使用12%的环氧乙烷、纸质包装和室温储存似乎会引起最显著的变化。在室温和常压下使用84%的环氧乙烷、聚乙烯包装材料以及在-20摄氏度下储存似乎能保护骨不脱水。随着失水量百分比降低（表明测试时脱水），骨的开裂和劈裂增加。由于灭菌和储存过程导致的脱水可能会导致在准备骨以插入骨螺钉时出现困难，进而危及使用此类同种异体植入物的修复稳定性。
