Physics Department, Federal University of Minas Gerais, C.P. 702, Belo Horizonte, MG 30123-970, Brazil.
Federal Center for Technological Education of Minas Gerais, Belo Horizonte, MG 30421-169, Brazil.
Biosensors (Basel). 2020 Dec 16;10(12):206. doi: 10.3390/bios10120206.
We present a graphene-based biosensor selective to recombinant cyanovirin-N (rCV-N), an antiviral protein that has proven to be an effective microbicide to inhibit HIV replication. We modified the graphene monolayer devices with 1-pyrenebutanoic acid succinimidyl ester, which interacts with both graphene and the primary and secondary amines of antibodies. By monitoring the change in the electrical resistance of the device, we were able to detect rCV-N in solutions in the range of 0.01 to 10 ng/mL, and found that the detection limit was 0.45 pg/mL, which is much smaller than that obtained with currently available techniques. This is important for applications of this microbicide against HIV, since it may be produced at a large scale from soya bean seeds processed using the available industrial processing technologies. The sensor showed high sensitivity, selectivity, and reproducibility.
我们提出了一种基于石墨烯的生物传感器，对重组氰病毒 N（rCV-N）具有选择性，rCV-N 是一种已被证明有效的抗 HIV 复制的杀菌剂。我们用 1- （ ） - 丁烷二酸琥珀酰亚胺酯对石墨烯单层器件进行修饰，该试剂与石墨烯以及抗体的伯胺和仲胺都有相互作用。通过监测器件电阻的变化，我们能够在 0.01 到 10ng/ml 的溶液范围内检测到 rCV-N，并发现检测限为 0.45pg/ml，这比目前可用的技术小得多。这对于这种杀菌剂针对 HIV 的应用很重要，因为它可以从使用现有工业加工技术加工的大豆种子中大规模生产。该传感器表现出高灵敏度、选择性和重现性。
