Department of Otorhinolaryngology, Yonsei University Wonju College of Medicine; Research Institute of Hearing Enhancement, Yonsei University Wonju College of Medicine; Department of Otorhinolaryngology Head and Neck Surgery, Hallym University College of Medicine.
Department of Otorhinolaryngology, Yonsei University Wonju College of Medicine; Research Institute of Hearing Enhancement, Yonsei University Wonju College of Medicine.
J Vis Exp. 2020 Dec 4(166). doi: 10.3791/61947.
To study the effects of mesenchymal stem cells (MSCs) on cell regeneration and treatment, this method tracks MSC migration and morphological changes after co-culture with cochlear epithelium. The organ of Corti was immobilized on a plastic coverslip by pressing a portion of the Reissner's membrane generated during the dissection. MSCs confined by a glass cylinder migrated toward cochlear epithelium when the cylinder was removed. Their predominant localization was observed in the modiolus of the organ of Corti, aligned in a direction similarly to that of the nerve fibers. However, some MSCs were localized in the limbus area and showed a horizontally elongated shape. In addition, migration into the hair cell area was increased, and the morphology of the MSCs changed to various forms after kanamycin treatment. In conclusion, the results of this study indicate that the coculture of MSCs with cochlear epithelium will be useful for the development of therapeutics via cell transplantation and for studies of cell regeneration that can examine various conditions and factors.
为了研究间充质干细胞（MSCs）对细胞再生和治疗的影响，该方法追踪了与耳蜗上皮细胞共培养后 MSC 的迁移和形态变化。通过按压解剖过程中产生的一部分 Reissner 膜，将 Corti 器官固定在塑料载玻片上。当玻璃圆筒被移除时，被其限制的 MSC 向耳蜗上皮细胞迁移。它们的主要定位观察到在 Corti 器官的中轴，排列方向与神经纤维相似。然而，一些 MSC 定位在嵴区域，并呈现水平拉长的形状。此外，进入毛细胞区域的迁移增加，并且在卡那霉素处理后 MSC 的形态发生了各种变化。总之，这项研究的结果表明，MSCs 与耳蜗上皮细胞的共培养将有助于通过细胞移植进行治疗，并有助于研究细胞再生，可以检查各种条件和因素。
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