Lei Bin, Zhang Shou-Bo, Xie Li-Xia, Mao Xiang-Ming
Department of Urology, The First Hospital Affiliated to Jinan University, Guangzhou, Guangdong 510630, China.
Center for Reproductive Medicine, Guangdong Armed Police Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510507, China.
Zhonghua Nan Ke Xue. 2020 Feb;26(2):128-133.
To investigate the expression of phosphoribosyl pyrophosphate synthase 2 (PRPS2) in the human testis and its clinical significance.
Using quantitative real-time PCR (qRT-PCR) and immunohistochemistry, we detected the expression of PRPS2 mRNA in the testis tissue of the men with normal spermatogenesis or mile, moderate or severe hypospermatogenesis (HS) and that of the PRPS2 protein in the testicular biopsy tissue of 67 adult males. Then, we analyzed the relationship of the PRPS2 expressions with the testicular histological types and clinical parameters of the subjects.
The expression of PRPS2 mRNA in the testis tissue was significantly higher in the normal spermatogenesis group than in the moderate and severe HS groups (P < 0.01). The positive expression of the PRPS2 protein was 70.0% in the normal spermatogenesis group, 66.7% in the mild HS group, 50.0% in the moderate HS group and 23.8% in the severe HS group, significantly higher in the normal spermatogenesis and mild HS groups than in the moderate and severe HS groups (P < 0.01). No significant correlation, however, was observed between the PRPS2 expression and clinical parameters of the subjects (P > 0.05).
PRPS2 is lowly expressed in the testis tissue of the men with hypospermatogenesis and its expression level may help the diagnosis of male infertility and the prediction of the spermatogenic function of the testis.
探讨磷酸核糖焦磷酸合成酶2（PRPS2）在人睾丸组织中的表达及其临床意义。
采用定量实时荧光定量聚合酶链反应（qRT-PCR）和免疫组织化学方法，检测正常生精或轻度、中度、重度生精功能低下（HS）男性睾丸组织中PRPS2 mRNA的表达，以及67例成年男性睾丸活检组织中PRPS2蛋白的表达。然后，分析PRPS2表达与受试者睾丸组织学类型和临床参数之间的关系。
正常生精组睾丸组织中PRPS2 mRNA的表达显著高于中度和重度HS组（P < 0.01）。正常生精组PRPS2蛋白阳性表达率为70.0%，轻度HS组为66.7%，中度HS组为50.0%，重度HS组为23.8%，正常生精组和轻度HS组显著高于中度和重度HS组（P < 0.01）。然而，未观察到PRPS2表达与受试者临床参数之间存在显著相关性（P > 0.05）。
PRPS2在生精功能低下男性的睾丸组织中低表达，其表达水平可能有助于男性不育症的诊断及睾丸生精功能的预测。
