Priority Research Centre Grow Up Well, Hunter Medical Research Institute, School of Medicine and Public Health, University of Newcastle, Callaghan, NSW 2305, Australia.
Priority Research Centre for Healthy Lungs, University of Newcastle and Hunter Medical Research Institute, Callaghan, NSW 2305, Australia.
Nutrients. 2020 Dec 18;12(12):3872. doi: 10.3390/nu12123872.
Measurement of vitamin D status has significant use in clinical and research settings, including during pregnancy. We aimed to assess the agreement of total 25-hydroxyvitamin D (25(OH)D) concentration, and its three analytes (25-hydroxyvitamin D (25(OH)D), 25-hydroxyvitamin D (25(OH)D) and Epi-25-hydroxyvitamin D (Epi-25(OH)D)), in plasma and serum samples collected during pregnancy, and to examine the proportion of women who change vitamin D status category based on sample type. Matching samples were collected from = 114 non-fasting women between 12-25 weeks gestation in a clinical trial in Newcastle, Australia. Samples were analysed by liquid chromatography-tandem mass-spectrometry (LC-MS/MS) to quantify total 25(OH)D and its analytes and examined using Bland-Altman plots, Pearson correlation (r), intraclass correlation coefficient and Cohen's Kappa test. Serum total 25(OH)D ranged from 33.8-169.8 nmol/L and plasma ranged from 28.6-211.2 nmol/L. There was a significant difference for total 25(OH)D based on sample type (measurement bias 7.63 nmol/L for serum vs plasma (95% Confidence Interval (CI) 5.36, 9.90, ≤ 0.001). The mean difference between serum and plasma concentrations was statistically significant for 25(OH)D (7.38 nmol/L; 95% CI 5.28, 9.48, ≤ 0.001) and Epi-25(OH)D (0.39 nmol/L; 95% CI 0.14, 0.64, = 0.014). Of 114 participants, 28% were classified as vitamin D deficient (<50 nmol/L) or insufficient (<75 nmol/L) based on plasma sample and 36% based on serum sample. Nineteen (16.7%) participants changed vitamin D status category based on sample type. 25-hydroxyvitamin D quantification using LC-MS/MS methodology differed significantly between serum and plasma, yielding a higher value in plasma; this influenced vitamin D status based on accepted cut-points, which may have implications in clinical and research settings.
在临床和研究环境中，包括在怀孕期间，维生素 D 状态的测量具有重要意义。我们旨在评估孕妇血浆和血清样本中总 25-羟维生素 D（25（OH）D）浓度及其三种分析物（25-羟维生素 D（25（OH）D）、25-羟维生素 D（25（OH）D）和 Epi-25-羟维生素 D（Epi-25（OH）D））的一致性，并检查基于样本类型的维生素 D 状态类别变化的女性比例。在澳大利亚纽卡斯尔的一项临床试验中，从 12-25 周妊娠的 114 名非禁食女性中采集了匹配样本。通过液相色谱-串联质谱法（LC-MS/MS）分析样本以定量总 25（OH）D 及其分析物，并使用 Bland-Altman 图、Pearson 相关系数（r）、组内相关系数和 Cohen's Kappa 检验进行检查。血清总 25（OH）D 范围为 33.8-169.8 nmol/L，血浆范围为 28.6-211.2 nmol/L。基于样本类型，总 25（OH）D 存在显著差异（血清与血浆之间的测量偏差为 7.63 nmol/L（95%置信区间（CI）为 5.36，9.90， ≤ 0.001）。血清和血浆浓度之间的平均差异在统计学上具有显著性，25（OH）D 为 7.38 nmol/L（95%CI 为 5.28，9.48， ≤ 0.001），Epi-25（OH）D 为 0.39 nmol/L（95%CI 为 0.14，0.64， = 0.014）。在 114 名参与者中，根据血浆样本，28%的人被归类为维生素 D 缺乏症（<50 nmol/L）或不足症（<75 nmol/L），根据血清样本，36%的人被归类为维生素 D 缺乏症或不足症。根据样本类型，19 名（16.7%）参与者的维生素 D 状态类别发生变化。使用 LC-MS/MS 方法学对 25-羟维生素 D 进行定量，在血浆中差异显著，产生更高的值；这影响了基于公认临界点的维生素 D 状态，这可能对临床和研究环境产生影响。
