Ferraresso Serena, Bargelloni Luca, Babbucci Massimiliano, Cannas Rita, Follesa Maria Cristina, Carugati Laura, Melis Riccardo, Cau Angelo, Koutrakis Manos, Sapounidis Argyrios, Crosetti Donatella, Patarnello Tomaso
Department of Comparative Biomedicine and Food Science, University of Padova, Legnaro, Padua 35020, Italy.
Department of Life and Environmental Sciences, University of Cagliari, Cagliari 09124, Italy.
iScience. 2020 Nov 30;24(1):101886. doi: 10.1016/j.isci.2020.101886. eCollection 2021 Jan 22.
Whole-genome sequencing data were produced from a single flathead grey mullet female and assembled into a draft genome sequence, whereas publicly available sequence data were used to obtain a male draft sequence. Two pools, each consisting of 60 unrelated individuals, respectively, of male and female fish were analyzed using Pool-Sequencing. Mapping and analysis of Pool-Seq data against the draft genome(s) revealed >30 loci potentially associated with sex, the most promising locus of which, encoding the () and harboring two missense variants, was genotyped on 245 fish from four Mediterranean populations. Genotype data showed that represents a previously unknown sex-determining locus, although the incomplete association pattern between genotype and sex-phenotype, the variability of such pattern across different populations, and the presence of other candidate loci reveal that a greater complexity underlies sex determination in the flathead grey mullet.
全基因组测序数据来自一条雌性鲻鱼，并组装成一个基因组草图序列，而公开可用的序列数据则用于获得雄性基因组草图序列。使用混合测序法对两个分别由60条无关的雄性和雌性鱼类个体组成的混合样本进行了分析。将混合测序数据与基因组草图进行比对和分析，发现了30多个可能与性别相关的位点，其中最有希望的位点编码（）并含有两个错义变体，对来自四个地中海种群的245条鱼进行了基因分型。基因型数据表明， 代表了一个以前未知的性别决定位点，尽管基因型与性别表型之间的关联模式不完整，这种模式在不同种群中的变异性，以及其他候选位点的存在表明，鲻鱼的性别决定存在更大的复杂性。
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