College of Plant Protection, Northwest A & F University, Yangling, Shaanxi 712100, China.
College of Plant Protection, Northwest A & F University, Yangling, Shaanxi 712100, China.
Pestic Biochem Physiol. 2021 Jan;171:104697. doi: 10.1016/j.pestbp.2020.104697. Epub 2020 Sep 2.
Glycoprotein (GP)-1 is a glycoprotein elicitor with antiviral activity found in Streptomyces kanasensis zx01. GP-1 can induce programmed cell death (PCD) in vitro; however, the underlying mechanism is unclear. In the present study, we demonstrated that GP-1 induced PCD in tobacco suspension cells, which was modulated by hydrogen peroxide (HO). GP-1 participated in and modulated biologically relevant signaling in plant cells. GP-1 induced tobacco cell death in a dose- and time-dependent manner; affected the expression of BRI1-associated receptor kinase 1 (BAK1) and the accumulation of salicylic acid (SA), which are related to PCD; and enzymatic activities and mitochondrial functions. In conclusion, GP-1-induced PCD in tobacco may be mediated by HO which alters BAK1 and SA levels, as well as mitochondrial and gene function. This cell signal cascade played an important role in the process of GP-1 induced plant disease resistance.
糖蛋白（GP）-1 是一种在链霉菌 kanasensis zx01 中发现的具有抗病毒活性的糖蛋白激发子。GP-1 可以在体外诱导程序性细胞死亡（PCD）；然而，其潜在机制尚不清楚。在本研究中，我们证明了 GP-1 在烟草悬浮细胞中诱导 PCD，这是由过氧化氢（HO）调节的。GP-1 参与并调节植物细胞中与生物学相关的信号转导。GP-1 以剂量和时间依赖的方式诱导烟草细胞死亡；影响与 PCD 相关的 BRI1 相关受体激酶 1（BAK1）和水杨酸（SA）的表达；以及酶活性和线粒体功能。总之，GP-1 诱导的烟草 PCD 可能是由 HO 介导的，HO 改变了 BAK1 和 SA 的水平，以及线粒体和基因功能。该细胞信号级联在 GP-1 诱导植物抗病性的过程中发挥了重要作用。
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