Laboratory of Neuroscience, School of Psychology, University of Colima, Colima, 28040, Mexico; Physiological Science PhD Program. School of Medicine, University of Colima, Colima, 28040, Mexico.
Department of Nanomaterials, Sustainable Chemistry Research Center, National Autonomous University of Mexico/Autonomous University of the State of Mexico, Toluca, 50200, Mexico.
Neurosci Res. 2021 Sep;170:76-86. doi: 10.1016/j.neures.2020.12.003. Epub 2021 Jan 6.
Gold nanoparticles (GNPs) have unique physical and chemical properties that allow them to function as a drug-delivery system for several tissues: skin, eye, liver, and others. However, information about the biological response of brain tissue against GNPs is limited. Astrocytes and microglia cells are the first line of defense against brain insults and proper indicators of the level of brain damage. This study was aimed to evaluate the astrocytic and microglia response after an intracerebral injection of polyethylene-glycol-coupled GNPs (PEGylated GNPs). We injected spherical PEGylated GNPs (85 × 10 nanoparticles /nl) with a glass micropipette (inner diameter =35 μm) into the striatum of P60 CD1 mice. We evaluated the cellular response of astrocytes and microglia on days 3, 7, 14, 30, and 90 after intracerebral injection. For both astrocytes and microglia cells, our findings indicated that the glial response was transient and mainly circumscribed to the injection site. This evidence suggests that PEGylated GNPs are well-tolerated by the neural tissue. Understanding the effects of GNPs in the adult brain is a crucial step to design proper pharmacological vehicles to deliver long-lasting drugs.
金纳米颗粒（GNPs）具有独特的物理和化学性质，可作为多种组织（皮肤、眼睛、肝脏等）的药物输送系统。然而，关于脑组织对 GNPs 的生物反应的信息有限。星形胶质细胞和小胶质细胞是大脑损伤的第一道防线，也是大脑损伤程度的适当指标。本研究旨在评估脑内注射聚乙二醇偶联 GNPs（PEGylated GNPs）后星形胶质细胞和小胶质细胞的反应。我们用玻璃微量移液器（内径=35μm）将球形 PEGylated GNPs（85×10 个纳米颗粒/nl）注入 P60 CD1 小鼠纹状体。我们在脑内注射后第 3、7、14、30 和 90 天评估星形胶质细胞和小胶质细胞的细胞反应。对于星形胶质细胞和小胶质细胞，我们的研究结果表明，神经胶质反应是短暂的，主要局限于注射部位。这一证据表明，PEGylated GNPs 被神经组织很好地耐受。了解 GNPs 在成年大脑中的作用是设计适当的药物输送载体以输送长效药物的关键步骤。
