Liao Xue-Ke, Yan Han-Lu, Wang Zhi-Yuan, Chen Cheng, He Dong, Wu Yue-Long, Chen Qiu-Wen
School of River and Ocean Engineering, Chongqing Jiaotong University, Chongqing 400074, China.
Center for Eco-Environmental Research, Nanjing Hydraulic Research Institute, Nanjing 210029, China.
Huan Jing Ke Xue. 2020 Dec 8;41(12):5509-5517. doi: 10.13227/j.hjkx.202005183.
There were significant differences in the working efficiency and mechanism of constructed wetlands between low temperature and suitable temperature conditions. This study designed a horizontal subsurface flow constructed wetland (HFCW) and a vertical subsurface flow constructed wetland (VFCW) to explore their performance differences in advanced treatment of sewage based on contaminant degradation analysis including the removal of organic matters, total nitrogen (TN), and total phosphorus (TP), as well as the analysis of microbial community structure. The results showed that when the COD concentration of influent was between 37.50 to 80.00 mg·L, the concentration of total nitrogen and total phosphorus were within the first level A criteria specified in the discharge standard of pollutants for municipal wastewater treatment plant at the continuous flow of 2 m·d:①Both HFCW and VFCW showed stable degradation ability of organic matter in influent and good resistance to high organic load. ②Supplementation of the carbon source significantly improved the nitrogen removal efficiency of two subsurface flow constructed wetlands. HFCW achieved the average removal rate of TN at 76.01%, and the average removal rate of TN by VFCW reached 71.69% after the carbon addition. In contrast, dosage of an external carbon source showed limited effect on phosphorus removal. Furthermore, it worked more effectively for performance improvement of HFCW than that of VFCW. ③The analysis of microbial community structure in wetland substrate and plant rhizosphere samples revealed that Proteobacteria, Firmicutes, and Verrucomicrobia were the dominant phylum in two series of wetland samples. For the dominant microbiota at the genus level, there were more significant differences in microbial community structure in wetland substrate samples than that in plant rhizosphere samples. , , and contributed the most to the differences between the microbial communities of HFCW and VFCW. Overall, the species diversity and abundance of microbial samples from VFCW was higher than those from HFCW.
低温与适宜温度条件下人工湿地的运行效率和机制存在显著差异。本研究设计了水平潜流人工湿地（HFCW）和垂直潜流人工湿地（VFCW），基于包括有机物、总氮（TN）和总磷（TP）去除在内的污染物降解分析以及微生物群落结构分析，探讨它们在污水深度处理中的性能差异。结果表明，当进水COD浓度在37.50至80.00mg·L之间，总氮和总磷浓度在城市污水处理厂污染物排放标准规定的一级A标准范围内，连续流量为2m·d时：①HFCW和VFCW对进水有机物均表现出稳定的降解能力，且对高有机负荷具有良好的抗性。②碳源的添加显著提高了两种潜流人工湿地的脱氮效率。添加碳源后，HFCW的TN平均去除率达到76.01%，VFCW的TN平均去除率达到71.69%。相比之下，外加碳源对除磷效果有限。此外，它对HFCW性能提升的效果比对VFCW更显著。③湿地基质和植物根际样品的微生物群落结构分析表明，变形菌门、厚壁菌门和疣微菌门是两个系列湿地样品中的优势菌门。对于属水平的优势微生物群，湿地基质样品中的微生物群落结构差异比植物根际样品中的更显著。 、 和 对HFCW和VFCW微生物群落差异的贡献最大。总体而言，VFCW微生物样品的物种多样性和丰度高于HFCW。
