Long Non-Coding RNA Knockdown Inhibits Tongue Squamous Cell Carcinoma Malignancy by Targeting microRNA-493/HMGA2.

PURPOSE

Long intergenic non-protein-coding RNA 00466 () promotes lung adenocarcinoma progression. Nonetheless, the expression and precise roles of in tongue squamous cell carcinoma (TSCC) remains uncertain and warrant further investigation. Hence, the present study aimed to examine the effects on the aggressive TSCC cell characteristics and to elucidate the potential underlying mechanisms.

METHODS

First, expression in TSCC was determined by reverse transcription-quantitative PCR. Subsequently, cell proliferation, apoptosis, migration, and invasion in vitro, as well as tumor growth in vivo were assessed to examine the effects on TSCC cells.

RESULTS

was upregulated in TSCC. This upregulation was notably associated with shorter overall TSCC patient survival. In vitro experiments indicated that depletion suppressed TSCC cell proliferation, migration and invasion, and promoted apoptosis. An in vivo experiment revealed that downregulation attenuated TSCC tumor growth in vivo. Mechanistic analysis revealed that functions as a microRNA-493 (miR-493) molecular sponge, a miRNA that targets high-mobility group AT-hook 2 () mRNA. upregulated HMGA2 in TSCC cells, and this phenomenon was regulated by the miR-493 sponge. Rescue experiments revealed a decrease in the miR-493/HMGA2 axis output, partially reversing the effects of downregulation on aggressive TSCC cell behavior.

CONCLUSION

These findings demonstrate that promotes TSCC cell oncogenicity in vitro and in vivo by upregulating the miR-493/HMGA2 axis output. These results may provide a new perspective and new insight into the molecular mechanisms of TSCC.