人体血浆中[3H]去甲肾上腺素的动力学：高效液相色谱法分离氧化铝提取物是否必要？

Plasma [3H]noradrenaline kinetics in man: is separation of alumina extracts by high performance liquid chromatography necessary?

McCance A J, Forfar J C

Department of Cardiovascular Medicine, John Radcliffe Hospital, Oxford, UK.

Clin Sci (Lond). 1988 Feb;74(2):151-4. doi: 10.1042/cs0740151.

Abstract

After an 80 min intravenous infusion of 1-[3H]noradrenaline ([3H]NA) in 19 subjects, the proportion of alumina-extractable 3H due to [3H]NA was 86 +/- 2% in arterial and 78 +/- 2% in venous plasma. [3H]Dihydroxy-phenylethyleneglycol ([3H]DHPG) accounted for 8 +/- 1% in arterial and 13 +/- 1% in venous plasma. [3H]Dihydroxymandelic acid ([3H]DOMA) was not detected. 2. No time-dependent change in the proportion of alumina-extractable 3H due to [3H]NA was seen in either arterial or venous plasma over this period. In venous plasma the proportion of alumina-extractable 3H due to [3H]DHPG increased slightly between 30 and 80 min, from 10 +/- 1% to 13 +/- 1%. 3. The results were unchanged with chronic beta-blockade and with either 1-[2,5,6-3H]NA or 1-[7,8-3H]NA. 4. After [3H]NA infusion in man the radioactivity recovered after alumina extraction is predominantly [3H]NA and thus can be used directly to determine [3H]NA kinetics without the need for metabolite separation.

摘要

对19名受试者静脉输注1-[³H]去甲肾上腺素（[³H]NA）80分钟后，动脉血浆中因[³H]NA导致的可被氧化铝提取的³H比例为86±2%，静脉血浆中为78±2%。[³H]二羟苯乙二醇（[³H]DHPG）在动脉血浆中占8±1%，在静脉血浆中占13±1%。未检测到[³H]二羟扁桃酸（[³H]DOMA）。2. 在这段时间内，动脉或静脉血浆中因[³H]NA导致的可被氧化铝提取的³H比例均未出现随时间的变化。在静脉血浆中，因[³H]DHPG导致的可被氧化铝提取的³H比例在30至80分钟之间略有增加，从10±1%增至13±1%。3. 慢性β受体阻滞剂以及使用1-[2,5,6-³H]NA或1-[7,8-³H]NA时，结果均无变化。4. 在人体输注[³H]NA后，氧化铝提取后回收的放射性主要是[³H]NA，因此可直接用于测定[³H]NA动力学，无需分离代谢物。