Mergenthaler H G, Brühl P, Dörmer P
Gesellschaft für Strahlen- und Umweltforschung, Institut für Experimentelle Hämatologie, München, Federal Republic of Germany.
Exp Hematol. 1988 Feb;16(2):145-9.
Hemopoiesis was analyzed in a miniaturized long-term culture of human bone marrow cells by quantifying the production of granulocyte-macrophage progenitor cells. As in the conventional long-term culture system, hemopoiesis was dependent on the presence of a marrow-derived adherent layer. Adipocytes proved to be essential for long-term proliferation. Optimal growth conditions were maintained by incubation in McCoy's medium supplemented with hydrocortisone, fetal calf serum, and horse serum. When calculated back to the volume of conventional cultures, the numbers and kinetics of nucleated cells and granulocyte-macrophage colony-forming cells were comparable in both culture systems. The microsystem is therefore suitable for performing multiple analyses on small samples of cells.
通过对粒细胞-巨噬细胞祖细胞的产生进行定量分析，在人骨髓细胞的小型长期培养中对造血作用进行了研究。与传统的长期培养系统一样，造血作用依赖于骨髓来源的贴壁层的存在。脂肪细胞被证明对长期增殖至关重要。通过在补充了氢化可的松、胎牛血清和马血清的 McCoy 培养基中培养来维持最佳生长条件。当换算回传统培养的体积时，两种培养系统中有核细胞和粒细胞-巨噬细胞集落形成细胞的数量和动力学是可比的。因此，该微系统适用于对小细胞样本进行多种分析。
