Larsson L, Mårdh P A
Acta Pathol Microbiol Scand Suppl. 1977(259):5-15.
Gas chromatography (GC) can be used to characterize microorganisms chemically. Microbial whole cells and cell extracts can be analysed by GC after being thermally or chemically degraded, i.e. pyrolysed or hydrolysed. Microbial metabolic products can be detected by GC analysis of culture media or the atmosphere above such media, i.e. headspace analysis. Pyrolysis products can be analysed direct, while hydrolysis products and non-volatile metabolites must be derivatized before being chromatographed. Differences in the chromatographic patterns can be used to differentiate between microorganisms. Detection of metabolic products in body fluids by means of GC constitutes a potential technique for the rapid diagnosis of infectious diseases.
气相色谱法（GC）可用于对微生物进行化学表征。微生物全细胞和细胞提取物在经过热降解或化学降解，即热解或水解后，可通过气相色谱法进行分析。微生物代谢产物可通过对培养基或培养基上方的空气进行气相色谱分析来检测，即顶空分析。热解产物可直接进行分析，而水解产物和非挥发性代谢物在进行色谱分析之前必须进行衍生化处理。色谱图模式的差异可用于区分不同的微生物。通过气相色谱法检测体液中的代谢产物是一种用于快速诊断传染病的潜在技术。
