Entomological Research Institute, Ayub Agricultural Research Institute, Faisalabad, Punjab, Pakistan; Department of Entomology, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya, Multan, Pakistan.
Entomological Research Institute, Ayub Agricultural Research Institute, Faisalabad, Punjab, Pakistan.
Chemosphere. 2021 Apr;269:129367. doi: 10.1016/j.chemosphere.2020.129367. Epub 2020 Dec 18.
Pyriproxyfen is a biorational insecticide from IGR family, used worldwide against several economic pests. To evaluate the risk of pyriproxyfen resistance in dusky cotton bug, Oxycarenus hyalinipennis Costa (Hemiptera: Lygaeidae), a major concern for cotton producers, and to formulate strategies effective to tackle resistance, a field collected population was selected with pyriproxyfen under laboratory conditions using seed-dip method. A resistant strain designated as Pyr-SEL (G) was developed after repeatedly selecting O. hyalinipennis with pyriproxyfen over eighteen generations. Thereafter, fitness costs, realized heritability (h) and cross-resistance were investigated. As a result of selection, Pyr-SEL (G) developed a very high level of resistance (resistance ratio = 464.23-fold) compared with the susceptible strain unselected over twenty generations Un-SEL (G). The Pyr-SEL (G) conferred strong cross-resistance to bifenthrin (146.59-fold), lambda-cyhalothrin (132.96-fold) and fenoxycarb (91.06-fold), whereas showed moderate cross-resistance to diafenthiuron (28.86-fold) and fipronil (22.73-fold). The h estimate was 0.16 in Pyr-SEL (G). The developmental duration of O. hyalinipennis pre-adult prolonged, but traits of λ, r and R reduced in Pyr-SEL (G) compared with the Un-SEL (G). Also, the population projection obtained lower population size for Pyr-SEL (G) than Un-SEL (G). Fitness studies revealed that high resistance development to pyriproxyfen lowered the relative fitness of Pyr-SEL (G) (R = 0.38) compared with the Un-SEL (G). These findings may be practically valuable in tackling O. hyalinipennis resistance for better pest management.
溴氰虫酰胺是一种 IGR 类生物杀虫剂，在全世界范围内被用于防治多种经济害虫。为了评估溴氰虫酰胺对棉盲蝽 Oxycarenus hyalinipennis Costa（半翅目：盲蝽科）的抗性风险，这是棉花种植者的主要关注点，并且制定有效的策略来应对抗性，我们在实验室条件下使用浸种法从田间采集了一个种群，并使用溴氰虫酰胺进行了选择。经过 18 代以上的反复选择，我们成功地选育出了一个对溴氰虫酰胺具有抗性的品系，命名为 Pyr-SEL（G）。此后，我们研究了其适合度代价、现实遗传力（h）和交互抗性。经过选择，与未经 20 多代选择的敏感品系 Un-SEL（G）相比，Pyr-SEL（G）表现出非常高的抗性（抗性倍数为 464.23 倍）。Pyr-SEL（G）对高效氯氟氰菊酯（146.59 倍）、高效氯氟氰菊酯（132.96 倍）和虫酰肼（91.06 倍）表现出很强的交互抗性，而对哒螨灵（28.86 倍）和氟虫腈（22.73 倍）表现出中等交互抗性。Pyr-SEL（G）的 h 估计值为 0.16。与 Un-SEL（G）相比，Pyr-SEL（G）的 O. hyalinipennis 预成虫发育时间延长，但 λ、r 和 R 等特征降低。此外，Pyr-SEL（G）的种群预测结果显示其种群规模小于 Un-SEL（G）。适合度研究表明，高抗溴氰虫酰胺导致 Pyr-SEL（G）的相对适合度降低（R=0.38），与 Un-SEL（G）相比。这些发现对于更好地管理棉盲蝽的抗性具有实际价值。
