Farshad Omid, Heidari Reza, Zamiri Mohammad Javad, Retana-Márquez Socorro, Khalili Meghdad, Ebrahimi Melika, Jamshidzadeh Akram, Ommati Mohammad Mehdi
Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Animal Science, Shiraz University, Shiraz, Iran.
Front Vet Sci. 2020 Dec 17;7:591558. doi: 10.3389/fvets.2020.591558. eCollection 2020.
Carbon-based nanomaterials possess a remarkably high potential for biomedical applications due to their physical properties; however, their detrimental effects on reproduction are also concerned. Several reports indicate the toxicity of carbon nanotubes (CNT); nevertheless, their impact on intracellular organelles in the male reproductive organs has not been fully elucidated. Herein, we report on the reprotoxicity of single-walled (SWCNT) and multi-walled carbon nanotubes (MWCN) on several intracellular events and histological criteria in pubertal male BALB/c mice orally treated with 0, 10, and 50 mg/kg/day doses for 5 weeks. Biomarkers of oxidative stress and mitochondrial functionality, histopathological alterations, and epididymal sperm characteristics were determined. Oral administration of CNTs at 10 and 50 mg/kg evoked a significant decrement in weight coefficient, sperm viability and motility, hypo-osmotic swelling (HOS) test, sperm count, mitochondrial dehydrogenase activity, ATP content, total antioxidant capacity, and GSH/GSSH ratio in the testis and epididymal spermatozoa. On the other hand, percent abnormal sperm, testicular and sperm TBARS contents, protein carbonylation, ROS formation, oxidized glutathione level, and sperm mitochondrial depolarization were considerably increased. Significant histopathological and stereological alterations in the testis occurred in the groups challenged with CNTs. The current findings indicated that oxidative stress and mitochondrial impairment might substantially impact CNTs-induced reproductive system injury and sperm toxicity. The results can also be used to establish environmental standards for CNT consumption by mammals, produce new chemicals for controlling the rodent populations, and develop therapeutic approaches against CNTs-associated reproductive anomalies in the males exposed daily to these nanoparticles.
基于碳的纳米材料因其物理特性在生物医学应用方面具有极高的潜力；然而，它们对生殖的有害影响也受到关注。几份报告指出了碳纳米管（CNT）的毒性；尽管如此，它们对雄性生殖器官细胞内细胞器的影响尚未完全阐明。在此，我们报告了单壁（SWCNT）和多壁碳纳米管（MWCN）对青春期雄性BALB/c小鼠的几种细胞内事件和组织学标准的生殖毒性，这些小鼠经口给予0、10和50 mg/kg/天的剂量，持续5周。测定了氧化应激和线粒体功能的生物标志物、组织病理学改变以及附睾精子特征。经口给予10和50 mg/kg的碳纳米管会导致睾丸和附睾精子的重量系数、精子活力和运动能力、低渗肿胀（HOS）试验、精子计数、线粒体脱氢酶活性、ATP含量、总抗氧化能力以及GSH/GSSH比值显著下降。另一方面，异常精子百分比、睾丸和精子TBARS含量、蛋白质羰基化、ROS形成、氧化型谷胱甘肽水平以及精子线粒体去极化显著增加。在用碳纳米管攻击的组中，睾丸出现了显著的组织病理学和体视学改变。目前的研究结果表明，氧化应激和线粒体损伤可能会对碳纳米管诱导的生殖系统损伤和精子毒性产生重大影响。这些结果还可用于制定哺乳动物碳纳米管消费的环境标准、生产控制啮齿动物种群的新化学品，以及开发针对每天接触这些纳米颗粒的男性中与碳纳米管相关的生殖异常的治疗方法。
