Faculty of Veterinary Medicine, Department of Forensic Medicine and Toxicology, Kafrelsheikh University, Kafrelsheikh, Egypt.
Department of Legal Medicine, Graduate School of Medicine, Gifu University, Gifu, Japan.
Biomarkers. 2021 Mar;26(2):168-173. doi: 10.1080/1354750X.2020.1871412. Epub 2021 Jan 21.
Chronological age estimation is a challenging marker in the field of forensic medicine. The current study aimed to investigate the accuracy of signal joint T-cell receptor rearrangement excision circles (sjTRECs) quantification and telomere length measurement as methods for estimating chronological age.
Telomere length was estimated in the DNA derived from the buccal cells through estimating the telomeric restriction fragment (TRF) length using Telo Telomere Length Assay while the sjTRECs quantification was carried out on DNA isolated from the blood samples using qPCR.
The TRF length was shortened with increased age ( = -0.722, < 0.001). The sjTRECs were also decreased with increased age ( = -0.831, < 0.001). Stronger coefficient and lower standard error of the estimate was obtained when multiple regression analysis for age prediction based on the values of both methods was applied ( = -0.876, < 0.001).
年龄推断是法医学领域极具挑战性的标志。本研究旨在探讨信号关节 T 细胞受体重组切除环 (sjTRECs) 定量和端粒长度测量作为年龄推断方法的准确性。
通过使用 Telo 端粒长度分析试剂盒从口腔细胞的 DNA 中估计端粒重复片段 (TRF) 长度来估计端粒长度，同时使用 qPCR 从血液样本中分离的 DNA 中进行 sjTRECs 定量。
TRF 长度随年龄增长而缩短 ( = -0.722， < 0.001)。sjTRECs 也随年龄增长而减少 ( = -0.831， < 0.001)。当基于两种方法的值应用多元回归分析进行年龄预测时，获得了更强的系数和更低的估计标准误差 ( = -0.876， < 0.001)。
Zotero 插件