LncRNA // Axis is a Potential Therapeutic Target for Non-Small Cell Lung Cancer.

BACKGROUND

Long non-coding RNA (lncRNA) is a key part of non-coding RNA, and more and more evidence has revealed that it plays a vital role in tumors. is a lncRNA discovered in the early stage. However, it is still unclear whether and play a regulatory role in lung cancer (LC). This research aimed to determine the biological function of / in non-small cell lung cancer (NSCLC).

MATERIALS AND METHODS

In order to research the function of in NSCLC, RT-PCR, Western blot, luciferase assay and RNA immunoprecipitation assay were used to determine the relationship between and . CCK8 test, cell migration and invasion test were used to explore the influence of on proliferation and metastasis of LC cells. Tumor allotransplantation was used to detect the influence of on the growth of LC.

RESULTS

The results revealed that was obviously enhanced in LC cell lines. Further functional analysis showed that low expression of obviously suppressed the growth, migration and invasion of NSCLC and facilitated cell apoptosis. Determination of luciferase reporter gene revealed that was the direct target of in LC. In addition, was called the direct target of , and / had saved the role of in NSCLC cells. Tumor allotransplantation experiments showed that knocking down could inhibit the growth of LC.

CONCLUSION

In summary, our results showed that the down-regulation of in NSCLC inhibited its growth, migration and invasion through the / axis.