Qin Zijin, Chang Qiang, Lei Chen, He Yunfan, Huang Zhiyong, Xing Malcolm, Lu Feng
Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
Department of Mechanical Engineering, University of Manitoba, Winnipeg, Manitoba, Canada.
ACS Biomater Sci Eng. 2019 Jul 8;5(7):3440-3447. doi: 10.1021/acsbiomaterials.8b00930. Epub 2019 Jun 12.
Adipose flap expansion using a tissue engineering chamber (TEC) presents a promising candidate for soft tissue regeneration by activating in situ adipose tissue regeneration. However, foreign body reaction (FBR) and capsular contracture caused by a silicone chamber limit large tissue reconstruction. Here, a hydrophilic and biodegradable film made of poly(ethylene glycol) diacrylate (PEG-da) with methacrylated gelatin (gelatin-MA) was presented between the host tissue and silicone chamber to tune the local wound and to prevent initiation of FBR. After a 60 day investigation, 6.1-fold-regenerated fat tissue was obtained from the PEG-gelatin group, whereas only 3-fold tissue was harvested from a silicone group. Histological staining demonstrated that the structure of the neo-formed adipose tissue in both groups was similar to mature adipose tissue. Noticeably, a more distinct and denser fibrous capsule was observed in the silicone group compared to the PEG-gelatin group. Immunohistochemistry of CD206 and TGF-β expression indicated less M2 macrophage infiltration and a minor inflammation reaction with PEG-gelatin assistance. Less collagen deposition and myofibroblast activation in the PEG-gelatin group were demonstrated via α-SMA and type I collagen staining. All these demonstrated that a biocompatible membrane supplement can attenuate capsule formation and contracture leading to a larger tissue regeneration through the TEC technique, which could lead to new perspectives to the relationship between materials-mattered FBR and tissue regeneration.
使用组织工程腔室（TEC）进行脂肪瓣扩张通过激活原位脂肪组织再生，为软组织再生提供了一个有前景的选择。然而，由硅胶腔室引起的异物反应（FBR）和包膜挛缩限制了大组织重建。在此，在宿主组织和硅胶腔室之间放置了一种由聚（乙二醇）二丙烯酸酯（PEG-da）与甲基丙烯酸化明胶（gelatin-MA）制成的亲水性可生物降解薄膜，以调节局部伤口并防止FBR的引发。经过60天的研究，PEG-明胶组获得了6.1倍再生的脂肪组织，而硅胶组仅收获了3倍的组织。组织学染色表明，两组中新形成的脂肪组织结构与成熟脂肪组织相似。值得注意的是，与PEG-明胶组相比，硅胶组观察到更明显且更致密的纤维囊。CD206和TGF-β表达的免疫组织化学表明，在PEG-明胶的辅助下，M2巨噬细胞浸润较少且炎症反应较轻。通过α-SMA和I型胶原染色证明，PEG-明胶组中胶原沉积和肌成纤维细胞活化较少。所有这些表明，生物相容性膜补充剂可以减轻包膜形成和挛缩，通过TEC技术导致更大的组织再生，这可能为材料相关的FBR与组织再生之间的关系带来新的视角。
