College of Oceanography, Hohai University, Nanjing, China.
Postdoctoral Innovation Practice Base, Jiangsu Shuixian Industrial Company Limited, Yangzhou, China.
J Fish Dis. 2021 Jun;44(6):675-687. doi: 10.1111/jfd.13329. Epub 2021 Jan 10.
As one of the piscine rhabdoviruses, Siniperca chuatsi rhabdovirus (SCRV) has caused considerable losses to mandarin fish aquaculture industry. RNA-seq, as efficient transcriptome research method, has been widely used to study the immune response of fish to pathogens. This study reported the effect of SCRV infection at 0, 24 and 60 hr on S. chuatsi at the transcriptome level. A total of 61,527 unigenes with high quality were obtained, and 3,095, 1,854 and 227 differentially expressed genes (DEGs) were labelled between the Sc24 and Sc0 groups, the Sc60 and Sc0 groups and the Sc60 and Sc24 groups, respectively. Genes involved in innate and adaptive immunity were highlighted. In Gene Ontology analysis, the DEGs that participated in immune response, innate immune response and the regulation of apoptotic process were identified as enriched classes. Kyoto Encyclopedia of Genes and Genomes pathway results indicated that most DEGs caused by SCRV infection were identified in the immune system (retinoic acid-inducible gene-I-like receptor/Toll-like receptor/nucleotide-binding oligomerization domain-like receptor/C-type lectin receptor signalling pathway), cellular processes, cell growth and death (p53 signalling pathway, cellular senescence, apoptosis and phagosome), and metabolism. Quantitative real-time PCR was used to further verify the expression levels of 15 immune-related DEGs. The transcriptome database obtained in this study provided further in-depth insight into the immune response of S. chuatsi against SCRV.
作为鱼类弹状病毒之一，翘嘴鳜虹彩病毒（Siniperca chuatsi rhabdovirus，SCRV）已对鳜鱼养殖业造成了巨大损失。RNA 测序作为一种高效的转录组研究方法，已被广泛用于研究鱼类对病原体的免疫反应。本研究报告了 SCRV 在 0、24 和 60 h 感染鳜鱼后对其转录组水平的影响。共获得高质量的 61527 个 unigenes，其中 Sc24 组和 Sc0 组、Sc60 组和 Sc0 组、Sc60 组和 Sc24 组之间分别有 3095、1854 和 227 个差异表达基因（differentially expressed genes，DEGs）被标记。涉及先天和适应性免疫的基因被突出显示。在基因本体论分析中，参与免疫反应、先天免疫反应和凋亡过程调节的 DEGs 被鉴定为富集类。京都基因与基因组百科全书通路结果表明，SCRV 感染引起的大多数 DEGs 被鉴定为免疫系统（视黄酸诱导基因-I 样受体/ Toll 样受体/核苷酸结合寡聚化结构域样受体/C 型凝集素受体信号通路）、细胞过程、细胞生长和死亡（p53 信号通路、细胞衰老、凋亡和吞噬体）和代谢。实时荧光定量 PCR 进一步验证了 15 个免疫相关 DEGs 的表达水平。本研究获得的转录组数据库为进一步深入了解鳜鱼对 SCRV 的免疫反应提供了依据。
